APPLICATION OF METABOLIC FLUX ANALYSIS IN METABOLIC ENGINEERING

Cited 21 time in webofscience Cited 0 time in scopus
  • Hit : 408
  • Download : 0
Metabolic flux analysis (MFA) is an important analytical technique to quantify intracellular metabolic fluxes as a consequence of all catalytic and transcriptional interactions. In systems metabolic engineering, MFA has played important role to understand cellular physiology under particular conditions and predict its metabolic capability after genetic or environmental perturbations. Two methods using optimization procedure, (13)C-based flux analysis and constraints-based flux analysis, have been used generally on the basis of stoichiometry of metabolic reactions and mass balances around intracellular metabolites under pseudo-steady state assumption. Practically, MFA has been applied to generate new knowledge on the biological system, analyze cellular physiology system-wide, and consequently design metabolic engineering strategies at a systems-level. In this chapter, we study the basic principle of MFA (more particularly constraints-based flux analysis), inspect the characteristics of several in silk algorithms developed for system-wide analysis of cellular metabolic fluxes, and discuss their applications.
Publisher
ELSEVIER ACADEMIC PRESS INC
Issue Date
2011
Language
English
Article Type
Review; Book Chapter
Keywords

GENE KNOCKOUT SIMULATION; GENOME-SCALE MODELS; ESCHERICHIA-COLI; BALANCE ANALYSIS; C-13-LABELING EXPERIMENTS; ADAPTIVE EVOLUTION; ENZYME-ACTIVITIES; HIGH-THROUGHPUT; THERMODYNAMIC CONSTRAINTS; SACCHAROMYCES-CEREVISIAE

Citation

SYNTHETIC BIOLOGY, PT B: COMPUTER AIDED DESIGN AND DNA ASSEMBLY Book Series: Methods in Enzymology, v.498, pp.67 - 93

ISSN
0076-6879
DOI
10.1016/B978-0-12-385120-8.00004-8
URI
http://hdl.handle.net/10203/94833
Appears in Collection
CBE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 21 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0