IMMOBILIZATION CAN IMPROVE THE STABILITY OF HYBRIDOMA ANTIBODY PRODUCTIVITY IN SERUM-FREE MEDIA
Hybridoma cells (S3H5/γ2bA2) were cultivated in spinner flasks with 1% serum media and serum-free media. Monoclonal antibody productivity was maintained in 1% serum media. However, cells in serum-free media showed a decrease in antibody productivity, and it completely disappeared in IMDM-based low protein medium. This loss of antibody productivity was not observed when the cells were immobilized in alginate beads. In fact, immobilization enhanced the specific MAb productivity.
- Publisher
- Wiley-VCH Verlag
- Issue Date
- 1990-12
- Language
- English
- Article Type
- Article
- Citation
BIOTECHNOLOGY AND BIOENGINEERING, v.36, no.10, pp.1049 - 1055
- ISSN
- 0006-3592
- Appears in Collection
- BS-Journal Papers(저널논문)
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