Label-free CMOS DNA sensor with on-chip noise reduction scheme for real-time dna quantification = 잡음 제거 회로가 집적된 단일 칩 실시간 유전물질 정량 측정 센서

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In this research, we have been proposed and demonstrated a label-free field-effect sensing array integrated with CMOS readout circuitry to detect the surface potential determined by the negative charge in deoxyribonucleic acid (DNA) molecules for real-time detection and quantification of the products generated by polymerase chain reaction (PCR) process. Moreover, we have explored a non-surface binding detection technique which is the measurements of DNA molecules without immobilizing them on the sensing surface for the real-time sequential monitoring of their concentrations because DNA detection method using immobilization and hybridization is not suitable for real-time measurement. For implementing field-effect sensing array with CMOS readout circuitry, we have proposed a BioFET with the top-layer metal (90×90 $\mu m^2$ native $Al_2O_3$/Al) electrode which is connected to the floating gate of the sensing transistor. This allows the device to be fully compatible with standard CMOS process. The sensing electrodes are easily formed by sharing the same passivation layer opening mask with the bonding pads pattern, so that no additional processes are required to fabricate the sensing devices. For direct measurement of the surface potential change due to DNA molecules, we have proposed an operational amplifier circuit scheme by replacing proposed BioFET in one of the input transistors in a differential input stage. The field-effect transistor connected to the sensing electrode is integrated into the differential input stage of the folded-cascode amplifier to configure a voltage follower. From this circuit configuration, we can acquire the output voltage equal to the surface potential of the sensing electrode. Furthermore, the proposed sensor includes an on-chip noise reduction circuitry for suppressing offset fixed pattern noise and 1/f noise of the devices. A prototype sensor has been fabricated using 1-㎛ double-poly double-metal standard CMOS process. In this...
Advisors
Kim, Lee-Supresearcher김이섭researcher
Description
한국과학기술원 : 전기및전자공학전공,
Publisher
한국과학기술원
Issue Date
2008
Identifier
295413/325007  / 020035043
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 전기및전자공학전공, 2008.2, [ x, 102 p. ]

Keywords

CMOS sensor; Non-surface binding method; Real-time DNA quantification; Label-free detection; Correlated double sampling; 상보형 금속 산화막 반도체 센서; 표면 부착 없는 방식; 실시간 유전물질 정량화; 표식 없는 검출; 상관 이중 샘플링; CMOS sensor; Non-surface binding method; Real-time DNA quantification; Label-free detection; Correlated double sampling; 상보형 금속 산화막 반도체 센서; 표면 부착 없는 방식; 실시간 유전물질 정량화; 표식 없는 검출; 상관 이중 샘플링

URI
http://hdl.handle.net/10203/35453
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=295413&flag=dissertation
Appears in Collection
EE-Theses_Ph.D.(박사논문)
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