Characterization of neuron signaling using microelectrode array combined with fast and precise cooling device for cryoanesthesia

Abstract

Cryoanesthesia technology is effective on treatment of a skin surface as a purely physical technique without side effects, and study of cryoanesthesia in cellular level are need to improve its technology but there has been a lack of its study so far. Here, we introduce a novel platform for cryo-neuromodulation, which is composed of a microelectrode array (MEA) system and a high-speed and precise probe type cooling device that provides localized cooling temperature. We investigate the potential of cryo-neuromodulation by exploring temperature conditions (temperature and duration time) that cause block and recovery of neuron signal without cell damages.