Development of fed-batch culture for the production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Aeromonas

Abstract

Newly screened Aeromonas hydrophila and Aeromonas salmonicida achromogenes produces copolymer consisted of 3-hydroxybutyrate (3HB) and 3-hydroxyhexanoate (3HHx). The characteristics of cell growth and polymer accumulation were examined using various carbon sources. P(3HB-co-3HHx) was produced only from lauric acid and oleic acid. The P(3HB-co-3HHx) content of 28.8 wt% could be obtained in flask culture. By applying the optimally designed nutrient feeding strategy in fed-batch culture of A. hydrophila with oleic acid, cell dry weight, P(3HB-co-3HHx) content, and the 3HHx fraction obtained in 43 h were 95.7 g/L, 45.2 wt% and 17 mol%, respectively, resulting in the productivity of 1.01 g PHA/L-h.