Functional analysis of RNA motifs essential for BC200 RNA-mediated translational regulation

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 168
  • Download : 0
Brain cytoplasmic 200 RNA (BC200 RNA) is proposed to ad as a local translational modulator by inhibiting translation after being targeted to neuronal dendrites. However, the mechanism by which BC200 RNA inhibits translation is not fully understood. Although a detailed functional analysis of RNA motifs is essential for understanding the BC200 RNA-mediated translation-inhibition mechanism, there is little relevant research on the subject. Here, we performed a systematic domain-dissection analysis of BC200 RNA to identify functional RNA motifs responsible for its translational-inhibition activity. Various RNA variants were assayed for their ability to inhibit translation of luciferase mRNA in vitro. We found that the 111-200-nucleotide region consisting of part of the Alu domain as well as the A/C-rich domain (consisting of both the A-rich and C-rich domains) is most effective for translation inhibition. Surprisingly, we also found that individual A-rich, A/C-rich, and Alu domains can enhance translation but at different levels for each domain, and that these enhancing effects manifest as cap-dependent translation.
Publisher
KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
Issue Date
2020-02
Language
English
Article Type
Article
Citation

BMB REPORTS, v.53, no.2, pp.94 - 99

ISSN
1976-6696
DOI
10.5483/BMBRep.2020.53.2.153
URI
http://hdl.handle.net/10203/273387
Appears in Collection
CH-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0