Functional analysis of RNA motifs essential for BC200 RNA-mediated translational regulation

Cited 1 time in webofscience Cited 0 time in scopus
  • Hit : 192
  • Download : 1
DC FieldValueLanguage
dc.contributor.authorJang, Seonghuiko
dc.contributor.authorShin, Heegwonko
dc.contributor.authorLee, Younghoonko
dc.date.accessioned2020-03-23T08:20:20Z-
dc.date.available2020-03-23T08:20:20Z-
dc.date.created2019-08-30-
dc.date.created2019-08-30-
dc.date.issued2020-02-
dc.identifier.citationBMB REPORTS, v.53, no.2, pp.94 - 99-
dc.identifier.issn1976-6696-
dc.identifier.urihttp://hdl.handle.net/10203/273387-
dc.description.abstractBrain cytoplasmic 200 RNA (BC200 RNA) is proposed to ad as a local translational modulator by inhibiting translation after being targeted to neuronal dendrites. However, the mechanism by which BC200 RNA inhibits translation is not fully understood. Although a detailed functional analysis of RNA motifs is essential for understanding the BC200 RNA-mediated translation-inhibition mechanism, there is little relevant research on the subject. Here, we performed a systematic domain-dissection analysis of BC200 RNA to identify functional RNA motifs responsible for its translational-inhibition activity. Various RNA variants were assayed for their ability to inhibit translation of luciferase mRNA in vitro. We found that the 111-200-nucleotide region consisting of part of the Alu domain as well as the A/C-rich domain (consisting of both the A-rich and C-rich domains) is most effective for translation inhibition. Surprisingly, we also found that individual A-rich, A/C-rich, and Alu domains can enhance translation but at different levels for each domain, and that these enhancing effects manifest as cap-dependent translation.-
dc.languageEnglish-
dc.publisherKOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY-
dc.titleFunctional analysis of RNA motifs essential for BC200 RNA-mediated translational regulation-
dc.typeArticle-
dc.identifier.wosid000518179300005-
dc.identifier.scopusid2-s2.0-85081109551-
dc.type.rimsART-
dc.citation.volume53-
dc.citation.issue2-
dc.citation.beginningpage94-
dc.citation.endingpage99-
dc.citation.publicationnameBMB REPORTS-
dc.identifier.doi10.5483/BMBRep.2020.53.2.153-
dc.identifier.kciidART002560618-
dc.contributor.localauthorLee, Younghoon-
dc.description.isOpenAccessY-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorA/C-rich domain-
dc.subject.keywordAuthorAlu domain-
dc.subject.keywordAuthorBC200 RNA-
dc.subject.keywordAuthorRNA motifs-
dc.subject.keywordAuthorTranslational regulation-
dc.subject.keywordPlusLONG NONCODING RNAS-
dc.subject.keywordPlusNEURAL BC1 RNA-
dc.subject.keywordPlusPOLY(A)-BINDING PROTEIN-
dc.subject.keywordPlusNEURONAL BC1-
dc.subject.keywordPlusIN-VITRO-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusSEQUENCE-
dc.subject.keywordPlusRECOGNITION-
dc.subject.keywordPlusLOCATION-
dc.subject.keywordPlusSYSTEMS-
Appears in Collection
CH-Journal Papers(저널논문)
Files in This Item
110907.pdf(842.94 kB)Download
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 1 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0