Cloning and Characterization of Mannheimia succiniciproducens MBEL55E Phosphoenolpyruvate Carboxykinase (pckA) Gene
A pckA gene encoding phosphoenolpyruvate carboxykinase (PEPCK) was cloned and sequenced from the succinic acid producing bacterium Mannheimia succiniciproducens MBEL55E. The gene encoded a 538 residue polypeptide with a calculated molecular mass of 58.8 kDa and a calculated pI of 5.03. The deduced amino acid sequence of the M. succiniciprodutens MBEL55E PEPCK was similar to those of all known ATP-dependent PEPCKS.
- Publisher
- 한국생물공학회
- Issue Date
- 2002-04
- Language
- Korean
- Citation
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.7, no.2, pp.95 - 99
- ISSN
- 1226-8372
- Appears in Collection
- CBE-Journal Papers(저널논문)
- Files in This Item
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