Influence of gluconeogenic phosphoenolpyruvate carboxykinase (PCK) expression on succinic acid fermentation in Escherichia coli under high bicarbonate condition

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The effects of amplifying the gluconeogenic phosphoenolpyruvate carboxykinase of Escherichia coli ((pck(Ec)) on succinic acid production in E. coli were examined under anaerobic condition. No significant increase in succinic acid production was observed in E coli overexpressing the pck(Ec) gene without supplementing NaHCO3 or MgCO3, On the other hand, succinic acid production was enhanced as the NaHCO3 concentration was increased. When 20 g/l of NaHCO3 was added, succinic acid production in recombinant E coli overexpressing PCK was 2.2-fold higher than that observed in the wild-type strain. It was concluded that the gluconeogenic pck(Ec) overexpression enabled E. coli to enhance succinic acid production only under the high bicarbonate supplementation condition.
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Issue Date
2006-09
Language
English
Article Type
Article
Citation

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.16, pp.1448 - 1452

ISSN
1017-7825
URI
http://hdl.handle.net/10203/22797
Appears in Collection
CBE-Journal Papers(저널논문)
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