Metabolic engineering of Corynebacterium glutamicum to overproduce L-arginine

Abstract

We report a metabolically engineered Corynebacterium glutamicum strain for L-arginineproduction. Random mutagenesis was first performed to improve tolerance to Larginine.Inactivation of arginine operon repressors, reinforced PPP flux, deletion of theNcgl1221 exporter gene, optimized expression levels of argF and carAB and argGHoperon overexpression resulted in 81 g/L of L-arginine production in a 1,500 Lbioreactor under fed-batch conditions. [This work was supported by the TechnologyDevelopment Program to Solve Climate Changes on Systems Metabolic Engineering forBiorefineries from the Ministry of Science, ICT and Future Planning (MSIP) through theNational Research Foundation (NRF) of Korea.]