Cellular production of chemicals is essential for sustainable chemical industry. We metabolically engineered Escherichia coli for production 1,3-diaminopropane (1,3-DAP), a monomer for polyamide. Comparison of heterologous C4 and C5 pathways for 1,3-DAP production by in silico flux analysis revealed that the C4 pathway employing Acinetobacter baumanniidat and ddc genes, encoding 2-ketoglutarate 4-aminotransferase and L-2,4-diaminobutanolate decarboxylase, respectively, was more efficient. In a strain having feedback resistant aspartokinases, the ppc and aspC genes were overexpressed to increase flux towards 1,3-DAP synthesis. Also, knocking out pfkA was found to increase 1,3-DAP production by applying 128 synthetic small RNAs. Overexpression of the ppc and aspC genes in the pfkA deleted strain resulted in even higher production of 1,3-DAP. Fed-batch fermentation of the final engineered E. coli strain allowed production of 13 g/L of 1,3-DAP in a glucose minimal medium. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation (NRF) of Korea ]