Production of medium-chain-length polyhydroxyalkanoates by high-cell-density cultivation of Pseudomonas putida under phosphorus limitation

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High-cell-density fed-batch cultures of Pseudomonas putida were carried out for the production of medium-chain-length polyhydroxyalkanoates (PHAs) using oleic acid as a carbon source. By employing an optimal feeding strategy without the limitation of any nutrient, a high cell concentration of 173 g/L was achieved, but the PHA concentration and PHA content were only 32.3 g/L and 18.7 wt%, respectively. To increase the PHA concentration and content, phosphorus limitation was applied during fed-bath culture by reducing the initial KH,PO, concentration. When the initial KH,PO, concentration was reduced to 4 g/L, cell concentration, PHA concentration, and PHA content obtained in 38 h were 141 g/L, 72.6 g/L, and 51.4 wt%, respectively, resulting in a high productivity of 1.91 g PHA/L per hour. (C) 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 68: 466-470, 2000.
Publisher
JOHN WILEY SONS INC
Issue Date
2000-05
Language
English
Article Type
Article
Citation

BIOTECHNOLOGY AND BIOENGINEERING, v.68, no.4, pp.466 - 470

ISSN
0006-3592
URI
http://hdl.handle.net/10203/20200
Appears in Collection
CBE-Journal Papers(저널논문)
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