대기압 플라즈마에 대한 암과 정상 세포 사이의 차별화된 반응

Abstract

Atmospheric pressure plasma treatment has gained much attention in biomedical applications due to its selective activation of certain cell types. In this study, we compare cancer to normal cells of mammary gland (MDA-MB-231 vs. MCF-10A) to investigate the cellular responses to plasma in a cancer dependent manner. When treated with atmospheric pressure plasma at the input voltage of 1000 V for 2 minutes, MDA-MB-231 started to detach from the extracellular matrix while normal MCF-10A remained intact. Similarly to the plasma treated cases, high concentration of H₂O₂ provoked MDA-MB-231 cells to detach from the substrate in a few minutes while MCF10A cells were minimally perturbed. Moreover, immunostaining results from both plasma treated samples and H₂O₂ treated samples share a lot of features, confirming that the underlying mechanism behind plasma induced detachment is most likely due to reactive oxygen species in the plasma.