Metabolic flux analysis with grouping reaction constraint based on genomic context and flux-converging pattern analyses

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 375
  • Download : 0
Flux balance analysis (FBA) of a genome-scale metabolic model allows calculation of intracellular metabolic fluxes by optimizing an objective function. However, FBA simulation without additional information has some limitations, such the inaccurate prediction of fluxes and existence of multiple solutions for an optimal objective value. Here, we report a strategy to accurately predict metabolic fluxes based on grouping reaction constraints that restrict the achievable flux ranges of grouped reactions by genomic context and flux-converging pattern analyses. FBA of Escherichia coli genome-scale metabolic model was carried under several genotypic and environmental conditions by applying these constraints, resulted in flux values that were in good agreement with the experimentally measured 13C-based fluxes.
Publisher
한국화학공학회
Issue Date
2011-10
Language
English
Citation

Annual Fall Meeting of KIChE 2011

URI
http://hdl.handle.net/10203/170022
Appears in Collection
CBE-Conference Papers(학술회의논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0