A new antisense oligonucleotide delivery system based on self-assembled ODN–PEG hybrid conjugate micelles
The conjugate of antisense c-raf oligonucleotide (ODN) and poly(ethylene glycol) (PEG) was synthesized for intracellular ODN delivery. When combined with polyethylenimine (PEI), the ODN-PEG conjugate self-associated to form polyelectrolyte complex micelles in aqueous solution. The effective hydrodynamic diameter of the micelles was ca. 70 nm with a narrow size distribution. Flow cytometry analysis indicated that the cellular uptake of the micelles by A2780 cells was much higher than that of ODN alone. The micelles also showed a superior antiproliferative activity against ovarian cancer cells in vitro and in vivo. (C) 2003 Elsevier B.V. All rights reserved.
- Publisher
- Elsevier Science Bv
- Issue Date
- 2003-12
- Language
- English
- Article Type
- Article; Proceedings Paper
- Keywords
POLYION COMPLEX MICELLES; C-RAF KINASE; BLOCK-COPOLYMER; NUCLEASE RESISTANCE; POLYETHYLENE-GLYCOL; POLYMERIC MICELLES; FUSOGENIC PEPTIDE; OVARIAN-CANCER; PLASMID DNA; IN-VIVO
- Citation
JOURNAL OF CONTROLLED RELEASE, v.93, no.2, pp.183 - 191
- ISSN
- 0168-3659
- Appears in Collection
- BS-Journal Papers(저널논문)
- Files in This Item
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