CHO cells in biotechnology for production of recombinant proteins: current state and further potential

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Recombinant Chinese hamster ovary cells (rCHO) cells have been the most commonly used mammalian host for large-scale commercial production of therapeutic proteins. Recent advances in cell culture technology for rCHO cells have achieved significant improvement in protein production leading to titer of more than 10 g/L to meet the huge demand from market needs. This achievement is associated with progression in the establishment of high and stable producer and the optimization of culture process including media development. In this review article, we focus on current strategies and achievements in cell line development, mainly in vector engineering and cell engineering, for high and stable protein production in rCHO cells. The approaches that manipulate various DNA elements for gene targeting by site-specific integration and cis-acting elements to augment and stabilize gene expression are reviewed here. The genetic modulation strategy by "direct" cell engineering with growth-promoting and/or productivity-enhancing factors and omics-based approaches involved in transcriptomics, proteomics, and metabolomics to pursue cell engineering are also presented.
Publisher
SPRINGER
Issue Date
2012-02
Language
English
Article Type
Review
Keywords

CHINESE-HAMSTER OVARY; MATRIX ATTACHMENT REGIONS; MEDIATED CASSETTE EXCHANGE; BUTYRATE-INDUCED APOPTOSIS; GENE INTEGRATION SYSTEM; LOW CULTURE TEMPERATURE; HUMAN ANTITHROMBIN-III; LACTIC-ACID FORMATION; FED-BATCH CULTURE; SERUM-FREE MEDIA

Citation

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.93, no.3, pp.917 - 930

ISSN
0175-7598
DOI
10.1007/s00253-011-3758-5
URI
http://hdl.handle.net/10203/97342
Appears in Collection
BS-Journal Papers(저널논문)
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