Engineering of Bacterial Exotoxins for Highly Efficient and Receptor-Specific Intracellular Delivery of Diverse Cargos

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The intracellular delivery of proteins with high efficiency in a receptor-specific manner is of great significance in molecular medicine and biotechnology, but remains a challenge. Herein, we present the development of a highly efficient and receptor-specific delivery platform for protein cargos by combining the receptor binding domain of Escherichia coli Shiga-like toxin and the translocation domain of Pseudomonas aeruginosa exotoxin A. We demonstrated the utility and efficiency of the delivery platform by showing a cytosolic delivery of diverse proteins both in vitro and in vivo in a receptor-specific manner. In particular, the delivery system was shown to be effective for targeting an intracellular protein and consequently suppressing the tumor growth in xenograft mice. The present platform can be widely used for intracellular delivery of diverse functional macromolecules with high efficiency in a receptor-specific manner. (C) 2016 Wiley Periodicals, Inc.
Publisher
WILEY-BLACKWELL
Issue Date
2016-08
Language
English
Article Type
Article
Keywords

PSEUDOMONAS EXOTOXIN; PROTECTIVE ANTIGEN; PROTEIN DELIVERY; ANTHRAX TOXIN; SHIGA TOXIN; B-SUBUNIT; CELLS; TRANSLOCATION; DOMAIN; TERMINUS

Citation

BIOTECHNOLOGY AND BIOENGINEERING, v.113, no.8, pp.1639 - 1646

ISSN
0006-3592
DOI
10.1002/bit.25935
URI
http://hdl.handle.net/10203/213945
Appears in Collection
BS-Journal Papers(저널논문)
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