DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ryou, Sang-Mi | ko |
dc.contributor.author | Park, Mira | ko |
dc.contributor.author | Kim, Jong-Myung | ko |
dc.contributor.author | Jeon, Che Ok | ko |
dc.contributor.author | Yun, Cheol-Hui | ko |
dc.contributor.author | Han, Seung Hyun | ko |
dc.contributor.author | Kim, Si Wouk | ko |
dc.contributor.author | Lee, Younghoon | ko |
dc.contributor.author | Kim, Sudeok | ko |
dc.contributor.author | Han, Min Su | ko |
dc.contributor.author | Bae, Jeehyeon | ko |
dc.contributor.author | Lee, Kangseok | ko |
dc.date.accessioned | 2013-03-11T08:29:09Z | - |
dc.date.available | 2013-03-11T08:29:09Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2011-11 | - |
dc.identifier.citation | JOURNAL OF BIOTECHNOLOGY, v.156, no.2, pp.89 - 94 | - |
dc.identifier.issn | 0168-1656 | - |
dc.identifier.uri | http://hdl.handle.net/10203/98795 | - |
dc.description.abstract | A prerequisite for the therapeutic use of small RNAs is the development of a method that can deliver them into animals. Previous studies have shown the capability of functionalized gold nanoparticles to serve as a general platform for loading and delivering DNA oligonucleotides and short hairpin RNAs (shRNAs) into cultured human cells. Here, we report the ability of the gold nanoparticle-assisted gene delivery system (AuNP-GDS) to deliver shRNA to a xenograft tumor in a mouse model. AuNP-GDS delivery of in vitro synthesized shRNA targeted to the Mcl-1L gene knocked down levels of Mcl-1L mRNA and protein by similar to 36% and similar to 26%, respectively, which were sufficient to induce apoptosis of the xenograft tumor cells and consequently inhibited the development of the tumor. We demonstrated that our lego-like AuNP-GDS, which can easily load and deliver shRNAs targeted to any gene of interest into living systems, can deliver shRNAs into xenograft tumors, leading to antitumor activity in an animal model. (C) 2011 Elsevier B. V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | GENE DELIVERY | - |
dc.subject | DRUG-DELIVERY | - |
dc.subject | MAMMALIAN-CELLS | - |
dc.subject | VIRAL VECTORS | - |
dc.subject | DNA | - |
dc.subject | THERAPY | - |
dc.subject | MAINTENANCE | - |
dc.subject | EXPRESSION | - |
dc.subject | CHEMISTRY | - |
dc.subject | BIOLOGY | - |
dc.title | Inhibition of xenograft tumor growth in mice by gold nanoparticle-assisted delivery of short hairpin RNAs against Mcl-1L | - |
dc.type | Article | - |
dc.identifier.wosid | 000296528600001 | - |
dc.identifier.scopusid | 2-s2.0-80054014120 | - |
dc.type.rims | ART | - |
dc.citation.volume | 156 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 89 | - |
dc.citation.endingpage | 94 | - |
dc.citation.publicationname | JOURNAL OF BIOTECHNOLOGY | - |
dc.contributor.localauthor | Lee, Younghoon | - |
dc.contributor.nonIdAuthor | Ryou, Sang-Mi | - |
dc.contributor.nonIdAuthor | Park, Mira | - |
dc.contributor.nonIdAuthor | Kim, Jong-Myung | - |
dc.contributor.nonIdAuthor | Jeon, Che Ok | - |
dc.contributor.nonIdAuthor | Yun, Cheol-Hui | - |
dc.contributor.nonIdAuthor | Han, Seung Hyun | - |
dc.contributor.nonIdAuthor | Kim, Si Wouk | - |
dc.contributor.nonIdAuthor | Kim, Sudeok | - |
dc.contributor.nonIdAuthor | Han, Min Su | - |
dc.contributor.nonIdAuthor | Bae, Jeehyeon | - |
dc.contributor.nonIdAuthor | Lee, Kangseok | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Gold nanoparticle-assisted gene delivery system (AuNP-GDS) | - |
dc.subject.keywordAuthor | Short hairpin RNAs (shRNAs) | - |
dc.subject.keywordAuthor | Xenograft tumor | - |
dc.subject.keywordAuthor | Mcl-1 | - |
dc.subject.keywordPlus | GENE DELIVERY | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | VIRAL VECTORS | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | MAINTENANCE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CHEMISTRY | - |
dc.subject.keywordPlus | BIOLOGY | - |
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