DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Mi Kyung | ko |
dc.contributor.author | Jee, Hye Ja | ko |
dc.contributor.author | Shin, Jennifer Hyunjong | ko |
dc.contributor.author | Noh, Minsoo | ko |
dc.contributor.author | Kim, Soo You | ko |
dc.contributor.author | Lee, Chang Hoon | ko |
dc.date.accessioned | 2013-03-11T07:09:02Z | - |
dc.date.available | 2013-03-11T07:09:02Z | - |
dc.date.created | 2012-03-08 | - |
dc.date.created | 2012-03-08 | - |
dc.date.issued | 2011-12 | - |
dc.identifier.citation | BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, v.1811, no.12, pp.1021 - 1029 | - |
dc.identifier.issn | 1388-1981 | - |
dc.identifier.uri | http://hdl.handle.net/10203/98614 | - |
dc.description.abstract | Sphingosylphosphorylcholine (SPC) is found at increased levels in the malignant ascites of tumor patients and induces perinuclear reorganization of keratin 8 (K8) filaments that contribute to the viscoelasticity of metastatic cancer cells. In this study, we investigated the role and molecular mechanisms of Tgase-2 in SPC-induced K8 phosphorylation and perinuclear reorganization in PANC-1 cells (PAN(WT)), and in PANC-1 cells that stably expressed shTgase-2 or Tgase-2 (PAN(shTg2) and PAN(Tg2)). SPC induces the expression of Tgase-2 in a time- and dose-dependent manner. Gene silencing of Tgase-2 or cystamine suppressed the SPC-induced phosphorylation and perinuclear reorganization of K8 and suppressed the SPC-induced migration of PANC-1 cells. An inhibitor of c-Jun N-terminal kinase (JNK), SP600125, suppressed the SPC-induced phosphorylation of serine 431 in K8 and keratin reorganization. Next, we examined the effect of Tgase-2 on JNK activation of serine 431 phosphorylation in K8. Tgase-2 gene silencing suppressed the expression of active form JNK (pJNK). Constitutive or tetracyclin-induced conditional expression of Tgase-2 increased the levels of RINK. Tgase-2 was coimmunoprecipitated with K8 and JNK. In addition, K8 was coimmunoprecipitated with Tgase-2 and JNK. JNK was also coimmunoprecipitated with K8 and Tgase-2. Overall, these results suggest that Tgase-2 is involved in SPC-induced phosphorylation and perinuclear reorganization of K8 by activating INK and forming a triple complex with K8 and JNK. Therefore, SPC-induced Tgase-2 might be a new target for modulating keratin reorganization, metastasis of cancer cells and JNK activation. (C) 2011 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | TISSUE TRANSGLUTAMINASE | - |
dc.subject | FILAMENT REORGANIZATION | - |
dc.subject | INTERMEDIATE-FILAMENTS | - |
dc.subject | SIGNALING PATHWAY | - |
dc.subject | CANCER METASTASIS | - |
dc.subject | DRUG-RESISTANCE | - |
dc.subject | PROTEIN-KINASE | - |
dc.subject | KAPPA-B | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | ORGANIZATION | - |
dc.title | Novel participation of transglutaminase-2 through c-Jun N-terminal kinase activation in sphingosylphosphorylcholine-induced keratin reorganization of PANC-1 cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000298521700003 | - |
dc.identifier.scopusid | 2-s2.0-80155135500 | - |
dc.type.rims | ART | - |
dc.citation.volume | 1811 | - |
dc.citation.issue | 12 | - |
dc.citation.beginningpage | 1021 | - |
dc.citation.endingpage | 1029 | - |
dc.citation.publicationname | BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS | - |
dc.contributor.localauthor | Shin, Jennifer Hyunjong | - |
dc.contributor.nonIdAuthor | Park, Mi Kyung | - |
dc.contributor.nonIdAuthor | Jee, Hye Ja | - |
dc.contributor.nonIdAuthor | Noh, Minsoo | - |
dc.contributor.nonIdAuthor | Kim, Soo You | - |
dc.contributor.nonIdAuthor | Lee, Chang Hoon | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Sphingosylphosphorylcholine | - |
dc.subject.keywordAuthor | Transglutaminase-2 | - |
dc.subject.keywordAuthor | Keratin-8 phosphorylation and reorganization | - |
dc.subject.keywordAuthor | c-Jun N-terminal kinase | - |
dc.subject.keywordAuthor | Migration | - |
dc.subject.keywordPlus | TISSUE TRANSGLUTAMINASE | - |
dc.subject.keywordPlus | FILAMENT REORGANIZATION | - |
dc.subject.keywordPlus | INTERMEDIATE-FILAMENTS | - |
dc.subject.keywordPlus | SIGNALING PATHWAY | - |
dc.subject.keywordPlus | CANCER METASTASIS | - |
dc.subject.keywordPlus | DRUG-RESISTANCE | - |
dc.subject.keywordPlus | PROTEIN-KINASE | - |
dc.subject.keywordPlus | KAPPA-B | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | ORGANIZATION | - |
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