DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Tae Jung | ko |
dc.contributor.author | Lee, SJ | ko |
dc.contributor.author | Park, JP | ko |
dc.contributor.author | Yang, Min Ho | ko |
dc.contributor.author | Choi, Jong Hyun | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2013-03-11T01:37:29Z | - |
dc.date.available | 2013-03-11T01:37:29Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2011-01 | - |
dc.identifier.citation | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.11, no.1, pp.402 - 407 | - |
dc.identifier.issn | 1533-4880 | - |
dc.identifier.uri | http://hdl.handle.net/10203/97940 | - |
dc.description.abstract | Bacterial cell surface layers (S-layers) are one of the most common outermost cell envelope components of prokaryotic organisms (Archaea and bacteria), which can self-assemble into two-dimensional (2D) crystalline arrays. Production and characterization of the bacterial S-layer protein (SLP) from Geobacillus stearothermophilus, a thermophilic bacterium, are demonstrated in this study. Based on this, purified SLPs were applied for wrapping around single-walled carbon nanotubes (CNTs) and applying as electrochemical sensing tools. For the large scale production of SLP, fed-batch culture of G. stearothermophilus was carried out by DO-stat strategy. Purified SLPs were characterized by atomic force microscopy (AFM). After recrystallization of purified SLPs with gold colloids, the formation of two-dimensional (2D) oblique lattice was observed by transmission electron microscopy (TEM). Metallic or near metallic characteristics of CNTs were measured by current voltage (I-V) analyzer. By high-cell density cultivation, cells grew to 10 g/l of dry cell weight in 65 h and the S-layer contents were achieved up to 40% of total proteins. The SLPs were purified to electrophoretic homogeneity and the molecular mass was estimated to be about 105 kDa. The purified SLPs were self-assembled and confirmed their hexagonal symmetry lattice structure. The SLP subunits were cross-linked to each other and to the underlying CNTs by non-covalent interaction, which was caused to change the electric current between natural CNTs and SLP-wrapped CNTs. One-dimensional structure and large aspect ratio of the functionalized CNTs may allow effective targeting of biomolecules by specific binding, such as protein-protein, DNA-DNA, and protein-ligand interaction. Bacterial SLP could be used as a biological template for immobilization molecular array, and provides new approaches for nanoelectronic biosensor applications. | - |
dc.language | English | - |
dc.publisher | AMER SCIENTIFIC PUBLISHERS | - |
dc.title | Characterization of a Bacterial Self-Assembly Surface Layer Protein and Its Application as an Electrical Nanobiosensor | - |
dc.type | Article | - |
dc.identifier.wosid | 000286344400064 | - |
dc.identifier.scopusid | 2-s2.0-79955856524 | - |
dc.type.rims | ART | - |
dc.citation.volume | 11 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 402 | - |
dc.citation.endingpage | 407 | - |
dc.citation.publicationname | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | - |
dc.identifier.doi | 10.1166/jnn.2011.3264 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Lee, SJ | - |
dc.contributor.nonIdAuthor | Park, JP | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Surface Layer Protein | - |
dc.subject.keywordAuthor | Single-Walled Carbon Nanotubes Array | - |
dc.subject.keywordAuthor | Self-Assembled Nanocomplex | - |
dc.subject.keywordAuthor | Streptavidin-Biotin Interaction | - |
dc.subject.keywordAuthor | Electronic Biosensor | - |
dc.subject.keywordPlus | STEAROTHERMOPHILUS ATCC 12980 | - |
dc.subject.keywordPlus | S-LAYER | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | CARBON NANOTUBES | - |
dc.subject.keywordPlus | ARRAYS | - |
dc.subject.keywordPlus | RECRYSTALLIZATION | - |
dc.subject.keywordPlus | MEMBRANES | - |
dc.subject.keywordPlus | LATTICES | - |
dc.subject.keywordPlus | DOMAIN | - |
dc.subject.keywordPlus | GOLD | - |
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