DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, TJ | ko |
dc.contributor.author | Bhang, SH | ko |
dc.contributor.author | La, WG | ko |
dc.contributor.author | Yang, HS | ko |
dc.contributor.author | Seong, JY | ko |
dc.contributor.author | Lee, Haeshin | ko |
dc.contributor.author | Im, GI | ko |
dc.contributor.author | Lee, SH | ko |
dc.contributor.author | Kim, BS | ko |
dc.date.accessioned | 2013-03-11T01:31:44Z | - |
dc.date.available | 2013-03-11T01:31:44Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2011-04 | - |
dc.identifier.citation | BIOTECHNOLOGY LETTERS, v.33, no.4, pp.829 - 836 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | http://hdl.handle.net/10203/97929 | - |
dc.description.abstract | Implantation of chondrocytes isolated from patients and expanded in number in vitro is being used to treat patients with cartilage injuries. However, chondrocytes de-differentiate during culture with several passages, and cartilage regenerated by implantation of de-differentiated chondrocytes may be suboptimal. Here, we show that a spinner-flask culture system induces formation of chondrocyte aggregates and redifferentiate de-differentiated chondrocytes. Spinner-flask cultures induced the aggregate formation of chondrocytes with passage 1 or 4. Importantly, spinner-flask cultures induced redifferentiation of the de-differentiated chondrocytes, as type I collagen expression was significantly lower and type II collagen expression was significantly higher in spinner flask-cultured chondrocytes than in monolayer-cultured chondrocytes. This system is easily scalable and could be feasible for clinical setting. | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.subject | II COLLAGEN | - |
dc.subject | MACROPOROUS MICROCARRIERS | - |
dc.subject | NASAL CHONDROCYTES | - |
dc.subject | IN-VITRO | - |
dc.subject | CARTILAGE | - |
dc.subject | EXPANSION | - |
dc.subject | EXPRESSION | - |
dc.subject | HYPOXIA | - |
dc.subject | SYSTEMS | - |
dc.title | Spinner-flask culture induces redifferentiation of de-differentiated chondrocytes | - |
dc.type | Article | - |
dc.identifier.wosid | 000288548900026 | - |
dc.identifier.scopusid | 2-s2.0-79952751395 | - |
dc.type.rims | ART | - |
dc.citation.volume | 33 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 829 | - |
dc.citation.endingpage | 836 | - |
dc.citation.publicationname | BIOTECHNOLOGY LETTERS | - |
dc.identifier.doi | 10.1007/s10529-010-0488-1 | - |
dc.contributor.localauthor | Lee, Haeshin | - |
dc.contributor.nonIdAuthor | Lee, TJ | - |
dc.contributor.nonIdAuthor | Bhang, SH | - |
dc.contributor.nonIdAuthor | La, WG | - |
dc.contributor.nonIdAuthor | Yang, HS | - |
dc.contributor.nonIdAuthor | Seong, JY | - |
dc.contributor.nonIdAuthor | Im, GI | - |
dc.contributor.nonIdAuthor | Lee, SH | - |
dc.contributor.nonIdAuthor | Kim, BS | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Chondrocyte | - |
dc.subject.keywordAuthor | De-differentiation | - |
dc.subject.keywordAuthor | Redifferentiation | - |
dc.subject.keywordAuthor | Spinner-flask culture | - |
dc.subject.keywordPlus | II COLLAGEN | - |
dc.subject.keywordPlus | MACROPOROUS MICROCARRIERS | - |
dc.subject.keywordPlus | NASAL CHONDROCYTES | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | CARTILAGE | - |
dc.subject.keywordPlus | EXPANSION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | HYPOXIA | - |
dc.subject.keywordPlus | SYSTEMS | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.