Long-term stability of cell micropatterns on poly((3-(methacryloylamino)propyl)-dimethyl(3-sulfopropyl)ammonium hydroxide)-patterned silicon oxide surfaces

Abstract

In this work we compared the long-term stability and integrity of cell patterns on newly reported zwitterionic poly((3-(methacryloylamino)propyl)dimethyl(3-sulfopropyl)ammonium hydroxide) (poly (MPDSAH)) films with those on widely used poly(poly(ethylene glycol) methyl ether methacrylate) (poly (PEGMEMA)) ones The micropatterns of both polymers were formed on a silicon oxide surface by a combination of micropattern generation of a photoresist vapor deposition of a silane-based polymerization initiator and surface-initiated atom transfer radical polymerization (SI-ATRP) of each monomer MPDSAH or PEGMEMA. The successful formation of the silane initiator SAMs and poly(MPDSAH) and poly(PEGMEMA) micropatterns was confirmed by X-ray photoelectron spectroscopy (XPS) and imaging ellipsometry Onto each substrate patterned with poly(MPDSAH) or poly(PEGMEMA) NIH 313 fibroblast cells were seeded and the cell micropatterns were generated by the selective adhesion of cells on the cell-adhesive region of the patterned surfaces The cell pattern formed on the poly(MPDSAH)-patterned surface was observed to have a superior ability of finely maintaining its original line-shaped structure up to for 20 days when compared with the cell pattern formed on the poly(PEGMEMA)-patterned surface In order to verify the relationship between the integrity of the cell micropatterns and the stability of the underlying non-biofouling polymer layers we also investigated the long-term stability of the polymer films themselves Immersed in the cell culture media for one month in the aid of ellipsometry contact goniometry and XPS (C) 2010 Elsevier Ltd All rights reserved