DC Field | Value | Language |
---|---|---|
dc.contributor.author | An, Dong-Shan | ko |
dc.contributor.author | Cui, Chang-Hao | ko |
dc.contributor.author | Lee, Hyung-Gwan | ko |
dc.contributor.author | Wang, Liang | ko |
dc.contributor.author | Kim, Sun-Chang | ko |
dc.contributor.author | Lee, Sung-Taik | ko |
dc.contributor.author | Jin, Fengxie | ko |
dc.contributor.author | Yu, Hong-Shan | ko |
dc.contributor.author | Chin, Young-Won | ko |
dc.contributor.author | Lee, Hyeong-Kyu | ko |
dc.contributor.author | Im, Wan-Taek | ko |
dc.contributor.author | Kim, Song-Gun | ko |
dc.date.accessioned | 2013-03-09T21:01:33Z | - |
dc.date.available | 2013-03-09T21:01:33Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2010-09 | - |
dc.identifier.citation | APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.76, pp.5827 - 5836 | - |
dc.identifier.issn | 0099-2240 | - |
dc.identifier.uri | http://hdl.handle.net/10203/97450 | - |
dc.description.abstract | A new beta-glucosidase from a novel strain of Terrabacter ginsenosidimutans (Gsoil 3082(T)) obtained from the soil of a ginseng farm was characterized, and the gene, bgpA (1,947 bp), was cloned in Escherichia coli. The enzyme catalyzed the conversion of ginsenoside Rb1 {3-O-[beta-D-glucopyranosyl-(1-2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl-(1-6)-beta-D-glucopyranosyl]-20(S)-protopanaxadiol} to the more pharmacologically active rare ginsenosides gypenoside XVII {3-O-beta-D-glucopyranosyl-20-O-[beta-D-glucopyranosyl-(1-6)-beta-D-glucopyranosyl]-20(S)-protopanaxadiol}, gypenoside LXXV {20-O-[beta-D-glucopyranosyl-(1-6)-beta-D-glucopyranosyl]-20(S)-protopanaxadiol}, and C-K [20-O-(beta-D-glucopyranosyl)-20(S)-protopanaxadiol]. A BLAST search of the bgpA sequence revealed significant homology to family 3 glycoside hydrolases. Expressed in E. coli, beta-glucosidase had apparent K(m) values of 4.2 +/- 0.8 and 0.14 +/- 0.05 mM and V(max) values of 100.6 +/- 17.1 and 329 +/- 31 mu mol . min(-1) . mg of protein(-1) against p-nitrophenyl-beta-D-glucopyranoside and Rb1, respectively. The enzyme catalyzed the hydrolysis of the two glucose moieties attached to the C-3 position of ginsenoside Rb1, and the outer glucose attached to the C-20 position at pH 7.0 and 37 degrees C. These cleavages occurred in a defined order, with the outer glucose of C-3 cleaved first, followed by the inner glucose of C-3, and finally the outer glucose of C-20. These results indicated that BgpA selectively and sequentially converts ginsenoside Rb1 to the rare ginsenosides gypenoside XVII, gypenoside LXXV, and then C-K. Herein is the first report of the cloning and characterization of a novel ginsenoside-transforming beta-glucosidase of the glycoside hydrolase family 3. | - |
dc.language | English | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.subject | ALPHA-L-ARABINOFURANOSIDASE | - |
dc.subject | SULFOLOBUS-SOLFATARICUS | - |
dc.subject | DEOXYRIBONUCLEIC-ACID | - |
dc.subject | BACTERIAL SYSTEMATICS | - |
dc.subject | GLYCOSIDE HYDROLASES | - |
dc.subject | PANAX-NOTOGINSENG | - |
dc.subject | COMPOUND K | - |
dc.subject | AIR SAMPLE | - |
dc.subject | SAPONINS | - |
dc.subject | RB-1 | - |
dc.title | Identification and Characterization of a Novel Terrabacter ginsenosidimutans sp nov beta-Glucosidase That Transforms Ginsenoside Rb1 into the Rare Gypenosides XVII and LXXV | - |
dc.type | Article | - |
dc.identifier.wosid | 000281288000019 | - |
dc.identifier.scopusid | 2-s2.0-77956563577 | - |
dc.type.rims | ART | - |
dc.citation.volume | 76 | - |
dc.citation.beginningpage | 5827 | - |
dc.citation.endingpage | 5836 | - |
dc.citation.publicationname | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | - |
dc.identifier.doi | 10.1128/AEM.00106-10 | - |
dc.contributor.localauthor | Kim, Sun-Chang | - |
dc.contributor.localauthor | Lee, Sung-Taik | - |
dc.contributor.nonIdAuthor | An, Dong-Shan | - |
dc.contributor.nonIdAuthor | Jin, Fengxie | - |
dc.contributor.nonIdAuthor | Yu, Hong-Shan | - |
dc.contributor.nonIdAuthor | Chin, Young-Won | - |
dc.contributor.nonIdAuthor | Lee, Hyeong-Kyu | - |
dc.contributor.nonIdAuthor | Kim, Song-Gun | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | ALPHA-L-ARABINOFURANOSIDASE | - |
dc.subject.keywordPlus | SULFOLOBUS-SOLFATARICUS | - |
dc.subject.keywordPlus | DEOXYRIBONUCLEIC-ACID | - |
dc.subject.keywordPlus | BACTERIAL SYSTEMATICS | - |
dc.subject.keywordPlus | GLYCOSIDE HYDROLASES | - |
dc.subject.keywordPlus | PANAX-NOTOGINSENG | - |
dc.subject.keywordPlus | COMPOUND K | - |
dc.subject.keywordPlus | AIR SAMPLE | - |
dc.subject.keywordPlus | SAPONINS | - |
dc.subject.keywordPlus | RB-1 | - |
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