A microfluidic in vitro cultivation system for mechanical stimulation of bovine embryos

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This work demonstrates a novel microfluidic in vitro cultivation system for embryos that improves their development using a partially constricted channel that mimics peristaltic muscle contraction. Conventional photolithography and a PDMS replica molding process were used to make straight or constricted microchannels. To investigate the effects of constriction geometry on embryonic development, different constriction widths of the channel were designed. Bovine embryos were loaded and incubated by simply placing them on a tilting machine to provide embryo movement via gravity. The fertilized embryos were cultivated on the microfluidic in vitro cultivation system until the blastocyst, hatching, or hatched blastocyst stages. To confirm the quality of blastocysts in the microfluidic channel, double staining was performed and compared with bovine embryos cultivated by the conventional droplet method. The proportion of eight-cell development among total embryos in the constricted channel (56.7 +/- 13.7%; mean +/- SD) was superior to that in the straight channel (23.9 +/- 11.0%). This suggests that the effect of constriction is vital for the early development of bovine embryos in assisted-reproduction research.
Publisher
WILEY-V C H VERLAG GMBH
Issue Date
2009-09
Language
English
Article Type
Article
Keywords

ASSISTED REPRODUCTION; ZONA-PELLUCIDA; MOUSE EMBRYOS; CELL-CULTURE; FERTILIZATION; OOCYTES; TECHNOLOGY; MATURATION; SPERM

Citation

ELECTROPHORESIS, v.30, pp.3276 - 3282

ISSN
0173-0835
DOI
10.1002/elps.200900157
URI
http://hdl.handle.net/10203/97353
Appears in Collection
BS-Journal Papers(저널논문)BiS-Journal Papers(저널논문)
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