DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wang, Liang | ko |
dc.contributor.author | Liu, Qing-Mei | ko |
dc.contributor.author | Sung, Bong-Hyun | ko |
dc.contributor.author | An, Dong-Shan | ko |
dc.contributor.author | Lee, Hyung-Gwan | ko |
dc.contributor.author | Kim, Song-Gun | ko |
dc.contributor.author | Kim, Sun-Chang | ko |
dc.contributor.author | Lee, Sung-Taik | ko |
dc.contributor.author | Im, Wan-Taek | ko |
dc.date.accessioned | 2013-03-09T19:47:12Z | - |
dc.date.available | 2013-03-09T19:47:12Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2011-11 | - |
dc.identifier.citation | JOURNAL OF BIOTECHNOLOGY, v.156, no.2, pp.125 - 133 | - |
dc.identifier.issn | 0168-1656 | - |
dc.identifier.uri | http://hdl.handle.net/10203/97330 | - |
dc.description.abstract | A new beta-glucosidase gene (bglSp) was cloned from the ginsenoside converting Sphingomonas sp. strain 2F2 isolated from the ginseng cultivating filed. The bglSp consisted of 1344 bp (447 amino acid residues) with a predicted molecular mass of 49,399 Da. A BLAST search using the bglSp sequence revealed significant homology to that of glycoside hydrolase superfamily 1. This enzyme was overexpressed in Escherichia coli BL21 (DE3) using a pET21-MBP (TEV) vector system. Overexpressed recombinant enzymes which could convert the ginsenosides Rb-1, Rb-2, Rc and Rd to the more pharmacological active rare ginsenosides gypenoside XVII, ginsenoside C-O, ginsenoside C-Mc(1) and ginsenoside F-2, respectively, were purified by two steps with Amylose-affinity and DEAE-Cellulose chromatography and characterized. The kinetic parameters for beta-glucosidase showed the apparent K-m and V-max values of 2.9 +/- 0.3 mM and 515.4 +/- 38.3 mu mol min(-1) mg of protein(-1) against p-nitrophenyl-beta-D-glucopyranoside. The enzyme could hydrolyze the outer C3 glucose moieties of ginsenosides Rb-1, Rb-2, Rc and Rd into the rare ginsenosides Gyp XVII, C-O, C-Mc(1) and F-2 quickly at optimal conditions of pH 5.0 and 37 degrees C. A little ginsenoside F-2 production from ginsenosides Gyp XVII, C-O, and C-Mc(1) was observed for the lengthy enzyme reaction caused by the side ability of the enzyme. Crown Copyright (C) 2011 Published by Elsevier B. V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | Elsevier | - |
dc.subject | PANAX-NOTOGINSENG | - |
dc.subject | TRITERPENE SAPONINS | - |
dc.subject | BIOACTIVE SAPONINS | - |
dc.subject | GINSENG SAPONINS | - |
dc.subject | TRANSFORMATION | - |
dc.subject | ROOT | - |
dc.subject | PURIFICATION | - |
dc.subject | RG(1) | - |
dc.subject | RB1 | - |
dc.title | Bioconversion of ginsenosides Rb1, Rb2, Rc and Rd by novel β-glucosidase hydrolyzing outer 3-O glycoside from Sphingomonas sp. 2F2: Cloning, expression, and enzyme characterization | - |
dc.type | Article | - |
dc.identifier.wosid | 000296528600006 | - |
dc.identifier.scopusid | 2-s2.0-80054036380 | - |
dc.type.rims | ART | - |
dc.citation.volume | 156 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 125 | - |
dc.citation.endingpage | 133 | - |
dc.citation.publicationname | JOURNAL OF BIOTECHNOLOGY | - |
dc.contributor.localauthor | Kim, Sun-Chang | - |
dc.contributor.localauthor | Lee, Sung-Taik | - |
dc.contributor.nonIdAuthor | Sung, Bong-Hyun | - |
dc.contributor.nonIdAuthor | An, Dong-Shan | - |
dc.contributor.nonIdAuthor | Lee, Hyung-Gwan | - |
dc.contributor.nonIdAuthor | Kim, Song-Gun | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Ginsenoside | - |
dc.subject.keywordAuthor | Bioconversion | - |
dc.subject.keywordAuthor | beta-Glucosidase | - |
dc.subject.keywordAuthor | Sphingomonas | - |
dc.subject.keywordPlus | PANAX-NOTOGINSENG | - |
dc.subject.keywordPlus | TRITERPENE SAPONINS | - |
dc.subject.keywordPlus | BIOACTIVE SAPONINS | - |
dc.subject.keywordPlus | GINSENG SAPONINS | - |
dc.subject.keywordPlus | TRANSFORMATION | - |
dc.subject.keywordPlus | ROOT | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | RG(1) | - |
dc.subject.keywordPlus | RB1 | - |
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