DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Sol | ko |
dc.contributor.author | Lee, Jungwoon | ko |
dc.contributor.author | Kim, Ja Young | ko |
dc.contributor.author | Lim, Bobae | ko |
dc.contributor.author | Shin, Eung-Kyun | ko |
dc.contributor.author | Han, Yong Mahn | ko |
dc.contributor.author | Kim, Sung-Su | ko |
dc.contributor.author | Song, Jin-Ho | ko |
dc.contributor.author | Kirn, Jungho | ko |
dc.date.accessioned | 2013-03-09T19:40:41Z | - |
dc.date.available | 2013-03-09T19:40:41Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2009-05 | - |
dc.identifier.citation | INTERNATIONAL JOURNAL OF CANCER, v.124, no.10, pp.2312 - 2322 | - |
dc.identifier.issn | 0020-7136 | - |
dc.identifier.uri | http://hdl.handle.net/10203/97321 | - |
dc.description.abstract | The EWS-Oct-4 protein is a chimeric molecule in which the amino terminal domain (NTD) of the EWS becomes fused to the carboxy terminal domain (CTD) of the Cict-4 transcription factor. It was identified in human bone and soft-tissue tumors associated with t(6;22)(p21;q12). Using in vitro and in vivo systems, we found that the EWS-Oct-4 protein self-associates. The major domains required for self-association mapped to the EWS NTD (amino acids 70-163) and the POU DNA-binding domain. EWS-Oct-4 protein also associated with EWS-Oct-4 (V351P), which contains a mutation in the POU DNA-binding domain. Using electrophoretic mobility shift assays, we found that the EWS-Oct-4 (V351P) mutant interfered with wild-type EWS-Oct-4 DNA-binding activity. In addition, we found that EWS-Oct-4-mediated transcriptional activation was inhibited by EWS-Oct-4 (V351P) protein in vivo. Thus, this mutation in the POU DNA-binding domain results in a dominant negative protein. These findings suggest that the biological functions of the EWS-Oct-4 oncogene can be modulated by the dominant negative mutant EWS-Oct-4 (V351P). (C) 2008 Wiley-Liss, Inc. | - |
dc.language | English | - |
dc.publisher | WILEY-LISS | - |
dc.subject | GERM-CELL TUMORS | - |
dc.subject | EWINGS-SARCOMA PROTEIN | - |
dc.subject | BREAST-CANCER CELLS | - |
dc.subject | RNA-POLYMERASE-II | - |
dc.subject | TRANSCRIPTION FACTOR | - |
dc.subject | EMBRYONIC GENES | - |
dc.subject | STEM-CELLS | - |
dc.subject | POU-DOMAIN | - |
dc.subject | OCT-4 | - |
dc.subject | EWS | - |
dc.title | Mutation in the DNA-binding domain of the EWS-Oct-4 oncogene results in dominant negative activity that interferes with EWS-Oct-4-mediated transactivation | - |
dc.type | Article | - |
dc.identifier.wosid | 000265353200007 | - |
dc.identifier.scopusid | 2-s2.0-64249141070 | - |
dc.type.rims | ART | - |
dc.citation.volume | 124 | - |
dc.citation.issue | 10 | - |
dc.citation.beginningpage | 2312 | - |
dc.citation.endingpage | 2322 | - |
dc.citation.publicationname | INTERNATIONAL JOURNAL OF CANCER | - |
dc.identifier.doi | 10.1002/ijc.24228 | - |
dc.contributor.localauthor | Han, Yong Mahn | - |
dc.contributor.nonIdAuthor | Kim, Sol | - |
dc.contributor.nonIdAuthor | Lee, Jungwoon | - |
dc.contributor.nonIdAuthor | Kim, Ja Young | - |
dc.contributor.nonIdAuthor | Lim, Bobae | - |
dc.contributor.nonIdAuthor | Shin, Eung-Kyun | - |
dc.contributor.nonIdAuthor | Kim, Sung-Su | - |
dc.contributor.nonIdAuthor | Song, Jin-Ho | - |
dc.contributor.nonIdAuthor | Kirn, Jungho | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | EWS-Oct-4 | - |
dc.subject.keywordAuthor | self-association | - |
dc.subject.keywordAuthor | dominant negative | - |
dc.subject.keywordAuthor | chromosomal translocation | - |
dc.subject.keywordAuthor | transactivation | - |
dc.subject.keywordAuthor | transformation | - |
dc.subject.keywordAuthor | bone and soft-tissue tumors | - |
dc.subject.keywordPlus | GERM-CELL TUMORS | - |
dc.subject.keywordPlus | EWINGS-SARCOMA PROTEIN | - |
dc.subject.keywordPlus | BREAST-CANCER CELLS | - |
dc.subject.keywordPlus | RNA-POLYMERASE-II | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | EMBRYONIC GENES | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | POU-DOMAIN | - |
dc.subject.keywordPlus | OCT-4 | - |
dc.subject.keywordPlus | EWS | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.