DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zhao, Jing | ko |
dc.contributor.author | Ohsumi, Toshiro K. | ko |
dc.contributor.author | Kung, Johnny T. | ko |
dc.contributor.author | Ogawa, Yuya | ko |
dc.contributor.author | Grau, Daniel J. | ko |
dc.contributor.author | Sarma, Kavitha | ko |
dc.contributor.author | Song, Ji-Joon | ko |
dc.contributor.author | Kingston, Robert E. | ko |
dc.contributor.author | Borowsky, Mark | ko |
dc.contributor.author | Lee, Jeannie T. | ko |
dc.date.accessioned | 2013-03-09T18:37:22Z | - |
dc.date.available | 2013-03-09T18:37:22Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2010-12 | - |
dc.identifier.citation | MOLECULAR CELL, v.40, no.6, pp.939 - 953 | - |
dc.identifier.issn | 1097-2765 | - |
dc.identifier.uri | http://hdl.handle.net/10203/97162 | - |
dc.description.abstract | Polycomb proteins play essential roles in stem cell renewal and human disease. Recent studies of HOX genes and X inactivation have provided evidence for RNA cofactors in Polycomb repressive complex 2 (PRC2). Here we develop a RIP-seq method to capture the PRC2 transcriptome and identify a genome-wide pool of >9000 PRC2-interacting RNAs in embryonic stem cells. The transcriptome includes antisense, intergenic, and promoter-associated transcripts, as well as many unannotated RNAs. A large number of transcripts occur within imprinted regions, oncogene and tumor suppressor loci, and stem cell-related bivalent domains. We provide evidence for direct RNA-protein interactions, most likely via the Ezh2 subunit. We also identify Gtl2 RNA as a PRC2 cofactor that directs PRC2 to the reciprocally imprinted Dlk1 coding gene. Thus, Polycomb proteins interact with a genonne-wide family of RNAs, some of which may be used as biomarkers and therapeutic targets for human disease. | - |
dc.language | English | - |
dc.publisher | CELL PRESS | - |
dc.subject | EMBRYONIC STEM-CELLS | - |
dc.subject | EZH2 HISTONE METHYLTRANSFERASE | - |
dc.subject | H3 LYSINE 27 | - |
dc.subject | NONCODING RNAS | - |
dc.subject | DEVELOPMENTAL REGULATORS | - |
dc.subject | REPRESSIVE COMPLEX | - |
dc.subject | CHROMATIN STATE | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | X-INACTIVATION | - |
dc.subject | GROUP PROTEINS | - |
dc.title | Genome-wide Identification of Polycomb-Associated RNAs by RIP-seq | - |
dc.type | Article | - |
dc.identifier.wosid | 000285823900012 | - |
dc.identifier.scopusid | 2-s2.0-78650253763 | - |
dc.type.rims | ART | - |
dc.citation.volume | 40 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 939 | - |
dc.citation.endingpage | 953 | - |
dc.citation.publicationname | MOLECULAR CELL | - |
dc.identifier.doi | 10.1016/j.molcel.2010.12.011 | - |
dc.contributor.localauthor | Song, Ji-Joon | - |
dc.contributor.nonIdAuthor | Zhao, Jing | - |
dc.contributor.nonIdAuthor | Ohsumi, Toshiro K. | - |
dc.contributor.nonIdAuthor | Kung, Johnny T. | - |
dc.contributor.nonIdAuthor | Ogawa, Yuya | - |
dc.contributor.nonIdAuthor | Grau, Daniel J. | - |
dc.contributor.nonIdAuthor | Sarma, Kavitha | - |
dc.contributor.nonIdAuthor | Kingston, Robert E. | - |
dc.contributor.nonIdAuthor | Borowsky, Mark | - |
dc.contributor.nonIdAuthor | Lee, Jeannie T. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | EMBRYONIC STEM-CELLS | - |
dc.subject.keywordPlus | EZH2 HISTONE METHYLTRANSFERASE | - |
dc.subject.keywordPlus | H3 LYSINE 27 | - |
dc.subject.keywordPlus | NONCODING RNAS | - |
dc.subject.keywordPlus | DEVELOPMENTAL REGULATORS | - |
dc.subject.keywordPlus | REPRESSIVE COMPLEX | - |
dc.subject.keywordPlus | CHROMATIN STATE | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | X-INACTIVATION | - |
dc.subject.keywordPlus | GROUP PROTEINS | - |
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