DC Field | Value | Language |
---|---|---|
dc.contributor.author | An, Sojin | ko |
dc.contributor.author | Yeo, Kwon Joo | ko |
dc.contributor.author | Jeon, Young Ho | ko |
dc.contributor.author | Song, Ji-Joon | ko |
dc.date.accessioned | 2013-03-09T17:49:17Z | - |
dc.date.available | 2013-03-09T17:49:17Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2011-03 | - |
dc.identifier.citation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.286, no.10, pp.8369 - 8374 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/10203/97040 | - |
dc.description.abstract | Absent, small, or homeotic disc1 (Ash1) is a trithorax group histone methyltransferase that is involved in gene activation. Although there are many known histone methyltransferases, their regulatory mechanisms are poorly understood. Here, we present the crystal structure of the human ASH1L catalytic domain, showing its substrate binding pocket blocked by a loop from the post-SET domain. In this configuration, the loop limits substrate access to the active site. Mutagenesis of the loop stimulates ASH1L histone methyltransferase activity, suggesting that ASH1L activity may be regulated through the loop from the post-SET domain. In addition, we show that human ASH1L specifically methylates histone H3 Lys-36. Our data implicate that there may be a regulatory mechanism of ASH1L histone methyltransferases. | - |
dc.language | English | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.subject | TRITHORAX-GROUP PROTEIN | - |
dc.subject | SET DOMAIN | - |
dc.subject | METHYLATION | - |
dc.subject | KINASE | - |
dc.subject | GENES | - |
dc.subject | PRODUCT | - |
dc.subject | REGION | - |
dc.subject | H3 | - |
dc.title | Crystal Structure of the Human Histone Methyltransferase ASH1L Catalytic Domain and Its Implications for the Regulatory Mechanism | - |
dc.type | Article | - |
dc.identifier.wosid | 000288013300065 | - |
dc.identifier.scopusid | 2-s2.0-79953139091 | - |
dc.type.rims | ART | - |
dc.citation.volume | 286 | - |
dc.citation.issue | 10 | - |
dc.citation.beginningpage | 8369 | - |
dc.citation.endingpage | 8374 | - |
dc.citation.publicationname | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.identifier.doi | 10.1074/jbc.M110.203380 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Song, Ji-Joon | - |
dc.contributor.nonIdAuthor | An, Sojin | - |
dc.contributor.nonIdAuthor | Yeo, Kwon Joo | - |
dc.contributor.nonIdAuthor | Jeon, Young Ho | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | TRITHORAX-GROUP PROTEIN | - |
dc.subject.keywordPlus | SET DOMAIN | - |
dc.subject.keywordPlus | METHYLATION | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordPlus | PRODUCT | - |
dc.subject.keywordPlus | REGION | - |
dc.subject.keywordPlus | H3 | - |
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