Enhanced Production of Human Serum Albumin by Fed-Batch Culture of Hansenula polymorpha with High-Purity Oxygen

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dc.contributor.authorYoun, Jong Kyuko
dc.contributor.authorShang, Longanko
dc.contributor.authorKim, Moon Ilko
dc.contributor.authorJeong, Chang Moonko
dc.contributor.authorChang, Ho Namko
dc.contributor.authorHahm, Moon Sunko
dc.contributor.authorRhee, Sang Kiko
dc.contributor.authorKang, Hyun Ahko
dc.date.accessioned2013-03-09T16:08:51Z-
dc.date.available2013-03-09T16:08:51Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2010-11-
dc.identifier.citationJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.20, no.11, pp.1534 - 1538-
dc.identifier.issn1017-7825-
dc.identifier.urihttp://hdl.handle.net/10203/96826-
dc.description.abstractFed-batch cultures of Hansenula polymorpha were studied to develop an efficient biosystem to produce recombinant human serum albumin (HSA). To comply with this purpose, we used a high-purity oxygen-supplying strategy to increase the viable cell density in a bioreactor and enhance the production of target protein. A mutant strain, H. polymorpha GOT7, was utilized in this study as a host strain in both 5-l and 30-l scale fermentors. To supply high-purity oxygen into a bioreactor, nearly 100% high-purity oxygen from a commercial bomb or higher than 93% oxygen available in situ from a pressure swing adsorption (PSA) oxygen generator was employed. Under the optimal fermentation of H. polymorpha with high-purity oxygen, the final cell densities and produced HSA concentrations were 24.6 g/l and 5.1 g/l in the 5-l fermentor, and 24.8 g/l and 4.5 g/l in the 30-l fermentor, respectively. These were about 2-10 times higher than those obtained in air-based fed-batch fermentations. The discrepancies between the 5-l and 30-l fermentors with air supply were presumably due to the higher contribution of surface aeration over submerged aeration in the 5-l fermentor. This study, therefore, proved the positive effect of high-purity oxygen in enhancing viable cell density as well as target recombinant protein production in microbial fermentations.-
dc.languageEnglish-
dc.publisherKOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY-
dc.subjectYEAST SACCHAROMYCES-CEREVISIAE-
dc.subjectCOPY-NUMBER-
dc.subjectSECRETION-
dc.subjectPROMOTER-
dc.subjectBINDING-
dc.subjectDL-1-
dc.titleEnhanced Production of Human Serum Albumin by Fed-Batch Culture of Hansenula polymorpha with High-Purity Oxygen-
dc.typeArticle-
dc.identifier.wosid000284866800011-
dc.identifier.scopusid2-s2.0-78649859386-
dc.type.rimsART-
dc.citation.volume20-
dc.citation.issue11-
dc.citation.beginningpage1534-
dc.citation.endingpage1538-
dc.citation.publicationnameJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY-
dc.identifier.doi10.4014/jmb.0909.09046-
dc.contributor.localauthorChang, Ho Nam-
dc.contributor.nonIdAuthorYoun, Jong Kyu-
dc.contributor.nonIdAuthorShang, Longan-
dc.contributor.nonIdAuthorKim, Moon Il-
dc.contributor.nonIdAuthorJeong, Chang Moon-
dc.contributor.nonIdAuthorHahm, Moon Sun-
dc.contributor.nonIdAuthorRhee, Sang Ki-
dc.contributor.nonIdAuthorKang, Hyun Ah-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorFed-batch culture-
dc.subject.keywordAuthorhigh cell density-
dc.subject.keywordAuthorhigh-purity oxygen fermentation-
dc.subject.keywordAuthorhuman serum albumin-
dc.subject.keywordAuthorHansenula polymorpha-
dc.subject.keywordPlusYEAST SACCHAROMYCES-CEREVISIAE-
dc.subject.keywordPlusCOPY-NUMBER-
dc.subject.keywordPlusSECRETION-
dc.subject.keywordPlusPROMOTER-
dc.subject.keywordPlusBINDING-
dc.subject.keywordPlusDL-1-
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