DC Field | Value | Language |
---|---|---|
dc.contributor.author | Youn, Jong Kyu | ko |
dc.contributor.author | Shang, Longan | ko |
dc.contributor.author | Kim, Moon Il | ko |
dc.contributor.author | Jeong, Chang Moon | ko |
dc.contributor.author | Chang, Ho Nam | ko |
dc.contributor.author | Hahm, Moon Sun | ko |
dc.contributor.author | Rhee, Sang Ki | ko |
dc.contributor.author | Kang, Hyun Ah | ko |
dc.date.accessioned | 2013-03-09T16:08:51Z | - |
dc.date.available | 2013-03-09T16:08:51Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2010-11 | - |
dc.identifier.citation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.20, no.11, pp.1534 - 1538 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | http://hdl.handle.net/10203/96826 | - |
dc.description.abstract | Fed-batch cultures of Hansenula polymorpha were studied to develop an efficient biosystem to produce recombinant human serum albumin (HSA). To comply with this purpose, we used a high-purity oxygen-supplying strategy to increase the viable cell density in a bioreactor and enhance the production of target protein. A mutant strain, H. polymorpha GOT7, was utilized in this study as a host strain in both 5-l and 30-l scale fermentors. To supply high-purity oxygen into a bioreactor, nearly 100% high-purity oxygen from a commercial bomb or higher than 93% oxygen available in situ from a pressure swing adsorption (PSA) oxygen generator was employed. Under the optimal fermentation of H. polymorpha with high-purity oxygen, the final cell densities and produced HSA concentrations were 24.6 g/l and 5.1 g/l in the 5-l fermentor, and 24.8 g/l and 4.5 g/l in the 30-l fermentor, respectively. These were about 2-10 times higher than those obtained in air-based fed-batch fermentations. The discrepancies between the 5-l and 30-l fermentors with air supply were presumably due to the higher contribution of surface aeration over submerged aeration in the 5-l fermentor. This study, therefore, proved the positive effect of high-purity oxygen in enhancing viable cell density as well as target recombinant protein production in microbial fermentations. | - |
dc.language | English | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.subject | YEAST SACCHAROMYCES-CEREVISIAE | - |
dc.subject | COPY-NUMBER | - |
dc.subject | SECRETION | - |
dc.subject | PROMOTER | - |
dc.subject | BINDING | - |
dc.subject | DL-1 | - |
dc.title | Enhanced Production of Human Serum Albumin by Fed-Batch Culture of Hansenula polymorpha with High-Purity Oxygen | - |
dc.type | Article | - |
dc.identifier.wosid | 000284866800011 | - |
dc.identifier.scopusid | 2-s2.0-78649859386 | - |
dc.type.rims | ART | - |
dc.citation.volume | 20 | - |
dc.citation.issue | 11 | - |
dc.citation.beginningpage | 1534 | - |
dc.citation.endingpage | 1538 | - |
dc.citation.publicationname | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.identifier.doi | 10.4014/jmb.0909.09046 | - |
dc.contributor.localauthor | Chang, Ho Nam | - |
dc.contributor.nonIdAuthor | Youn, Jong Kyu | - |
dc.contributor.nonIdAuthor | Shang, Longan | - |
dc.contributor.nonIdAuthor | Kim, Moon Il | - |
dc.contributor.nonIdAuthor | Jeong, Chang Moon | - |
dc.contributor.nonIdAuthor | Hahm, Moon Sun | - |
dc.contributor.nonIdAuthor | Rhee, Sang Ki | - |
dc.contributor.nonIdAuthor | Kang, Hyun Ah | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Fed-batch culture | - |
dc.subject.keywordAuthor | high cell density | - |
dc.subject.keywordAuthor | high-purity oxygen fermentation | - |
dc.subject.keywordAuthor | human serum albumin | - |
dc.subject.keywordAuthor | Hansenula polymorpha | - |
dc.subject.keywordPlus | YEAST SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | COPY-NUMBER | - |
dc.subject.keywordPlus | SECRETION | - |
dc.subject.keywordPlus | PROMOTER | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | DL-1 | - |
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