Direct Generation of Neurosphere-Like Cells from Human Dermal Fibroblasts

Cited 11 time in webofscience Cited 0 time in scopus
  • Hit : 556
  • Download : 587
DC FieldValueLanguage
dc.contributor.authorLee, Soon-Taeko
dc.contributor.authorChu, Konko
dc.contributor.authorJung, Keun-Hwako
dc.contributor.authorSong, Young-Miko
dc.contributor.authorJeon, Daejongko
dc.contributor.authorKim, Seung U.ko
dc.contributor.authorKim, Manhoko
dc.contributor.authorLee, Sang Kunko
dc.contributor.authorRoh, Jae-Kyuko
dc.date.accessioned2013-03-09T10:03:10Z-
dc.date.available2013-03-09T10:03:10Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2011-07-
dc.identifier.citationPLOS ONE, v.6, no.7-
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/10203/96049-
dc.description.abstractNeural stem cell (NSC) transplantation replaces damaged brain cells and provides disease-modifying effects in many neurological disorders. However, there has been no efficient way to obtain autologous NSCs in patients. Given that ectopic factors can reprogram somatic cells to be pluripotent, we attempted to generate human NSC-like cells by reprograming human fibroblasts. Fibroblasts were transfected with NSC line-derived cellular extracts and grown in neurosphere culture conditions. The cells were then analyzed for NSC characteristics, including neurosphere formation, gene expression patterns, and ability to differentiate. The obtained induced neurosphere-like cells (iNS), which formed daughter neurospheres after serial passaging, expressed neural stem cell markers, and had demethylated SOX2 regulatory regions, all characteristics of human NSCs. The iNS had gene expression patterns that were a combination of the patterns of NSCs and fibroblasts, but they could be differentiated to express neuroglial markers and neuronal sodium channels. These results show for the first time that iNS can be directly generated from human fibroblasts. Further studies on their application in neurological diseases are warranted.-
dc.languageEnglish-
dc.publisherPUBLIC LIBRARY SCIENCE-
dc.subjectNEURAL STEM-CELLS-
dc.subjectHUMAN SOMATIC-CELLS-
dc.subjectADULT HUMAN BRAIN-
dc.subjectDEFINED FACTORS-
dc.subjectPROGENITOR CELLS-
dc.subjectNEURONS-
dc.subjectTRANSPLANTATION-
dc.subjectDIFFERENTIATION-
dc.subjectINDUCTION-
dc.subjectMOUSE-
dc.titleDirect Generation of Neurosphere-Like Cells from Human Dermal Fibroblasts-
dc.typeArticle-
dc.identifier.wosid000292781500017-
dc.identifier.scopusid2-s2.0-79960244419-
dc.type.rimsART-
dc.citation.volume6-
dc.citation.issue7-
dc.citation.publicationnamePLOS ONE-
dc.contributor.localauthorJeon, Daejong-
dc.contributor.nonIdAuthorLee, Soon-Tae-
dc.contributor.nonIdAuthorChu, Kon-
dc.contributor.nonIdAuthorJung, Keun-Hwa-
dc.contributor.nonIdAuthorSong, Young-Mi-
dc.contributor.nonIdAuthorKim, Seung U.-
dc.contributor.nonIdAuthorKim, Manho-
dc.contributor.nonIdAuthorLee, Sang Kun-
dc.contributor.nonIdAuthorRoh, Jae-Kyu-
dc.description.isOpenAccessY-
dc.type.journalArticleArticle-
dc.subject.keywordPlusNEURAL STEM-CELLS-
dc.subject.keywordPlusHUMAN SOMATIC-CELLS-
dc.subject.keywordPlusADULT HUMAN BRAIN-
dc.subject.keywordPlusDEFINED FACTORS-
dc.subject.keywordPlusPROGENITOR CELLS-
dc.subject.keywordPlusNEURONS-
dc.subject.keywordPlusTRANSPLANTATION-
dc.subject.keywordPlusDIFFERENTIATION-
dc.subject.keywordPlusINDUCTION-
dc.subject.keywordPlusMOUSE-
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 11 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0