DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Sungyoung | ko |
dc.contributor.author | Song, Seungjeong | ko |
dc.contributor.author | Choi, Chulhee | ko |
dc.contributor.author | Park, Je-Kyun | ko |
dc.date.accessioned | 2013-03-09T07:26:28Z | - |
dc.date.available | 2013-03-09T07:26:28Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2009-03 | - |
dc.identifier.citation | ANALYTICAL CHEMISTRY, v.81, no.5, pp.1964 - 1968 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | http://hdl.handle.net/10203/95727 | - |
dc.description.abstract | Cell cycle studies for examining regulatory mechanisms and progression invariably require synchronization of cell cultures at a specific phase of the cell cycle. Current implementations to produce synchronous cell populations, however, tend to perturb normal cellular progression and metabolism and typically require complex, time-consuming preparations. Thus, it is challenging for the development of a simple, noninvasive, and effective means for cell cycle synchronization. We demonstrate the use of hydrophoretic size separation to sort cells in tar-get phases of the cell cycle entirely based on a hydrodynamic principle. With this method, we found that there is a linear relationship between a cell's size and its position distribution in the hydrophoretic device. We also demonstrate the robustness of the hydrophoretic method for practical applications by sorting cells in the G(0)/G(1) and G(2)/M phases out of the original, asynchronous cells with a high level of synchrony of 95.5% and 85.2%, respectively. These results show that the hydrophoretic size separation can be used in order to collect cells at the same phase of the cell cycle in a gentle, noninvasive way. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | CAPILLARY-ELECTROPHORESIS | - |
dc.subject | CENTRIFUGAL ELUTRIATION | - |
dc.subject | SERUM STARVATION | - |
dc.subject | MICROCHANNEL | - |
dc.subject | NOCODAZOLE | - |
dc.subject | OBSTACLES | - |
dc.subject | CANCER | - |
dc.title | Microfluidic self-sorting of mammalian cells to achieve cell cycle synchrony by hydrophoresis | - |
dc.type | Article | - |
dc.identifier.wosid | 000263765100038 | - |
dc.identifier.scopusid | 2-s2.0-64749084287 | - |
dc.type.rims | ART | - |
dc.citation.volume | 81 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 1964 | - |
dc.citation.endingpage | 1968 | - |
dc.citation.publicationname | ANALYTICAL CHEMISTRY | - |
dc.identifier.doi | 10.1021/ac8024575 | - |
dc.contributor.localauthor | Choi, Chulhee | - |
dc.contributor.localauthor | Park, Je-Kyun | - |
dc.contributor.nonIdAuthor | Choi, Sungyoung | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | CAPILLARY-ELECTROPHORESIS | - |
dc.subject.keywordPlus | CENTRIFUGAL ELUTRIATION | - |
dc.subject.keywordPlus | SERUM STARVATION | - |
dc.subject.keywordPlus | MICROCHANNEL | - |
dc.subject.keywordPlus | NOCODAZOLE | - |
dc.subject.keywordPlus | OBSTACLES | - |
dc.subject.keywordPlus | CANCER | - |
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