DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이현진 | ko |
dc.contributor.author | 최석진 | ko |
dc.contributor.author | 서태석 | ko |
dc.contributor.author | 원종인 | ko |
dc.date.accessioned | 2013-03-09T00:58:35Z | - |
dc.date.available | 2013-03-09T00:58:35Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2010-04 | - |
dc.identifier.citation | KSBB JOURNAL, v.25, no.2, pp.199 - 204 | - |
dc.identifier.issn | 1225-7117 | - |
dc.identifier.uri | http://hdl.handle.net/10203/94894 | - |
dc.description.abstract | We generated the feasibility of DNA separation in free-solution using genetically engineered repetitive polypeptides as drag-tags. Two different-sized repetitive polypeptides were designed, expressed in E. coli, and purified. They were conjugated to a fluorescently labeled DNA (100 base), and the electrophoretic mobilities of these conjugate molecules were analyzed on a microchip. The results of these studies indicate that genetically engineered repetitive polypeptide is a prominent candidate for rapid and high-throughput genetic mutation detection, such as SNP analysis. | - |
dc.language | Korean | - |
dc.publisher | 한국생물공학회 | - |
dc.title | Microchip상에서 효율적인 DNA 분석을 위한 반복단위 단백질의 생산 | - |
dc.title.alternative | Production of Repetitive Polypeptides for an Efficient DNA Analysis on a Microchip | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.citation.volume | 25 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 199 | - |
dc.citation.endingpage | 204 | - |
dc.citation.publicationname | KSBB JOURNAL | - |
dc.identifier.kciid | ART001450849 | - |
dc.contributor.localauthor | 서태석 | - |
dc.contributor.nonIdAuthor | 이현진 | - |
dc.contributor.nonIdAuthor | 최석진 | - |
dc.contributor.nonIdAuthor | 원종인 | - |
dc.subject.keywordAuthor | repetitive polypeptide | - |
dc.subject.keywordAuthor | end-labeled free-solution electrophoresis (ELFSE) | - |
dc.subject.keywordAuthor | drag-tag | - |
dc.subject.keywordAuthor | capillary electrophoresis | - |
dc.subject.keywordAuthor | microchip | - |
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