DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Joon Seok | ko |
dc.contributor.author | Ryu, Jungki | ko |
dc.contributor.author | Park, Chan Beum | ko |
dc.date.accessioned | 2009-06-15T09:47:39Z | - |
dc.date.available | 2009-06-15T09:47:39Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2009-04 | - |
dc.identifier.citation | ANALYTICAL CHEMISTRY, v.81, no.7, pp.2751 - 2759 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | http://hdl.handle.net/10203/9432 | - |
dc.description.abstract | The principal histopathological feature of Alzheimer's disease is the presence of beta-amyloid (A beta) aggregates in the gray matter of the brain, and researchers believe that various environmental factors play significant roles in the conformational change and self-assembly of A beta peptides. Therefore, discovering a rapid and convenient analytical method of evaluating the environmental factors on A beta aggregation would have a considerable impact. Herein we report our development of a novel microfluidic screening system enabling high-throughput analysis, low-consumption of reagents, and short analytical time. Microchannels with a cross-sectional. dimension of 100 mu m x 100 mu m were immobilized with A beta monomers via N-hydroxysuccinimide ester activation of the internal surfaces, and then a fresh A beta monomer solution mixed with different small molecules or metal ions was continuously introduced into the microchannels to induce A beta aggregation. In this work, we investigated (1) the temporal evolution of A beta aggregation within microchannels, (2) the high-throughput screening of the inhibitory effect of 12 small molecules against A beta aggregation, and (3) the effect of different metal ions (Fe(3+), Cu(2+), Zn(2+), and Al(3+)) on A beta aggregation by using thioflavin T (ThT)-induced fluorescence microscopy and ex situ atomic force microscopy. The microfluidic system should contribute to a simultaneous analysis of multiple environmental factors affecting amyloid aggregates in a parallel manner and to screen therapeutic small molecules prior to their in vivo evaluation. | - |
dc.description.sponsorship | This research was supported by Grants from the BioGreen 21 Program (20070301034038) and the Korea Science and Engineering Foundation (KOSEF) National Research Laboratory (NRL) program (R0A-2008-000-20041-0). This research was also partially supported by a Grant from the Eco-Technopia 21 project (010-081-036) from the Ministry of Environment, Republic of Korea | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | Amer Chemical Soc | - |
dc.subject | FIBRILS IN-VITRO | - |
dc.subject | SOLID-SURFACE | - |
dc.subject | PROTEIN AGGREGATION | - |
dc.subject | METAL-IONS | - |
dc.subject | DISEASE | - |
dc.subject | PEPTIDE | - |
dc.subject | NEUROTOXICITY | - |
dc.subject | A-BETA-42 | - |
dc.subject | FIBRILLOGENESIS | - |
dc.subject | THERAPEUTICS | - |
dc.title | High-Throughput Analysis of Alzheimer's beta-Amyloid Aggregation Using a Microfluidic Self-Assembly of Monomersf | - |
dc.type | Article | - |
dc.identifier.wosid | 000264759400045 | - |
dc.identifier.scopusid | 2-s2.0-64849106509 | - |
dc.type.rims | ART | - |
dc.citation.volume | 81 | - |
dc.citation.issue | 7 | - |
dc.citation.beginningpage | 2751 | - |
dc.citation.endingpage | 2759 | - |
dc.citation.publicationname | ANALYTICAL CHEMISTRY | - |
dc.identifier.doi | 10.1021/ac802701z | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Park, Chan Beum | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | FIBRILS IN-VITRO | - |
dc.subject.keywordPlus | SOLID-SURFACE | - |
dc.subject.keywordPlus | PROTEIN AGGREGATION | - |
dc.subject.keywordPlus | METAL-IONS | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | PEPTIDE | - |
dc.subject.keywordPlus | NEUROTOXICITY | - |
dc.subject.keywordPlus | A-BETA-42 | - |
dc.subject.keywordPlus | FIBRILLOGENESIS | - |
dc.subject.keywordPlus | THERAPEUTICS | - |
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