Human nuclear clusterin mediates apoptosis by interacting with Bcl-XL through C-terminal coiled coil domain

Cited 48 time in webofscience Cited 0 time in scopus
  • Hit : 420
  • Download : 0
Clusterin (CLU), a glycoprotein, is involved in apoptosis, producing two alternatively spliced isoforms in various cell types. The pro-apoptotic CLU appears to be a nuclear isoform (nuclear clusterin; nCLU), and the secretory CLU (sCLU) is thought to be anti-apoptotic. The detailed molecular mechanism of nCLU as a pro-apoptotic molecule has not yet been clear. In the current study, overexpressed nCLU induced apoptosis in human kidney cells. Biochemical studies revealed that nCLU sequestered Bcl-XL via a putative BH3 motif in the C-terminal coiled coil (CC2) domain, releasing Bax, and promoted apoptosis accompanied by activation of caspase-3 and cytochrome c release. These results suggest a novel mechanism of apoptosis mediated by nCLU as a pro-apoptotic molecule. J. Cell. Physiol. 227: 11571167, 2012. (C) 2011 Wiley Periodicals, Inc.
Publisher
WILEY-BLACKWELL
Issue Date
2012-03
Language
English
Article Type
Article
Keywords

PROSTATE-CANCER CELLS; MITOCHONDRIAL APOPTOSIS; BH3-ONLY PROTEINS; PEPTIDE COMPLEX; BH3 DOMAINS; FAMILY-MEMBERS; TNF-ALPHA; BAX; DEATH; MCL-1

Citation

JOURNAL OF CELLULAR PHYSIOLOGY, v.227, no.3, pp.1157 - 1167

ISSN
0021-9541
DOI
10.1002/jcp.22836
URI
http://hdl.handle.net/10203/93964
Appears in Collection
BiS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 48 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0