DC Field | Value | Language |
---|---|---|
dc.contributor.author | Diao, Jiajie | ko |
dc.contributor.author | Ishitsuka, Yuji | ko |
dc.contributor.author | Bae, Woo-Ri | ko |
dc.date.accessioned | 2013-03-08T17:14:20Z | - |
dc.date.available | 2013-03-08T17:14:20Z | - |
dc.date.created | 2012-03-08 | - |
dc.date.created | 2012-03-08 | - |
dc.date.issued | 2011-12 | - |
dc.identifier.citation | BIOSCIENCE REPORTS, v.31, no.6, pp.457 - 463 | - |
dc.identifier.issn | 0144-8463 | - |
dc.identifier.uri | http://hdl.handle.net/10203/93706 | - |
dc.description.abstract | Membrane fusion is one of the most important cellular processes by which two initially distinct lipid bilayers merge their hydrophobic cores, resulting in one interconnected structure. Proteins, called SNARE (soluble N-ethylmaleimide-sensitive factor-attachment protein receptor), play a central role in the fusion process that is also regulated by several accessory proteins. In order to study the SNARE-mediated membrane fusion, the in vitro protein reconstitution assay involving ensemble FRET (fluorescence resonance energy transfer) has been used over a decade. In this mini-review, we describe several single-molecule-based FRET approaches that have been applied to this field to overcome the shortage of the bulk assay in terms of protein and fusion dynamics. | - |
dc.language | English | - |
dc.publisher | PORTLAND PRESS LTD | - |
dc.subject | ALTERNATING-LASER EXCITATION | - |
dc.subject | RESONANCE ENERGY-TRANSFER | - |
dc.subject | VESICLE FUSION | - |
dc.subject | COMPLEXIN BINDING | - |
dc.subject | FLUORESCENCE | - |
dc.subject | PROTEINS | - |
dc.subject | SYNAPTOTAGMIN | - |
dc.subject | RESOLUTION | - |
dc.subject | REVEALS | - |
dc.subject | DRIVEN | - |
dc.title | Single-molecule FRET study of SNARE-mediated membrane fusion | - |
dc.type | Article | - |
dc.identifier.wosid | 000298718600002 | - |
dc.identifier.scopusid | 2-s2.0-84155176609 | - |
dc.type.rims | ART | - |
dc.citation.volume | 31 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 457 | - |
dc.citation.endingpage | 463 | - |
dc.citation.publicationname | BIOSCIENCE REPORTS | - |
dc.contributor.nonIdAuthor | Diao, Jiajie | - |
dc.contributor.nonIdAuthor | Ishitsuka, Yuji | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Review | - |
dc.subject.keywordAuthor | fluorescence resonance energy transfer (FRET) | - |
dc.subject.keywordAuthor | membrane fusion | - |
dc.subject.keywordAuthor | single molecule | - |
dc.subject.keywordAuthor | soluble N-ethylmaleimide-sensitive factor-attachment protein receptor (SNARE) | - |
dc.subject.keywordPlus | ALTERNATING-LASER EXCITATION | - |
dc.subject.keywordPlus | RESONANCE ENERGY-TRANSFER | - |
dc.subject.keywordPlus | VESICLE FUSION | - |
dc.subject.keywordPlus | COMPLEXIN BINDING | - |
dc.subject.keywordPlus | FLUORESCENCE | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | SYNAPTOTAGMIN | - |
dc.subject.keywordPlus | RESOLUTION | - |
dc.subject.keywordPlus | REVEALS | - |
dc.subject.keywordPlus | DRIVEN | - |
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