DC Field | Value | Language |
---|---|---|
dc.contributor.author | IM, Seung-Soon | ko |
dc.contributor.author | Kwon, Sool-Ki | ko |
dc.contributor.author | Kang, Seung-Youn | ko |
dc.contributor.author | Kim, Tae-Hyun | ko |
dc.contributor.author | Kim, Hail | ko |
dc.contributor.author | Hur, Man-Wook | ko |
dc.contributor.author | Kim, Kyung-Sup | ko |
dc.contributor.author | Ahn, Yong-Ho | ko |
dc.date.accessioned | 2013-03-08T11:45:14Z | - |
dc.date.available | 2013-03-08T11:45:14Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2006-10 | - |
dc.identifier.citation | BIOCHEMICAL JOURNAL, v.399, pp.131 - 139 | - |
dc.identifier.issn | 0264-6021 | - |
dc.identifier.uri | http://hdl.handle.net/10203/92933 | - |
dc.description.abstract | Expression of the GLUT4 (glucose transporter type 4 isoform) gene in adipocytes is subject to hormonal or metabolic control. In the present study, we have characterized an adipose tissue transcription factor that is influenced by fasting/refeeding regimens and insulin. Northern blotting showed that refeeding increased GLUT4 mRNA levels for 24 h in adipose tissue. Consistent with an increased GLUT4 gene expression, the mRNA levels of SREBP (sterol-regulatory-element-binding protein)-1c in adipose tissue were also increased by refeeding. In streptozotocin-induced diabetic rats, insulin treatment increased the mRNA levels of GLUT4 in adipose tissue. Serial deletion, luciferase reporter assays and electrophoretic mobility-shift assay studies indicated that the putative sterol response element is located in the region between bases - 109 and - 100 of the human GLUT4 promoter. Transduction of the SREBP-1c dominant negative form to differentiated 3T3-L1 adipocytes caused a reduction in the mRNA levels of GLUT4, suggesting that SREBP-1c mediates the transcription of GLUT4. In vivo chromatin immunoprecipitation revealed that refeeding increased the binding of SREBP-1 to the putative sterol-response element in the GLUT4. Furthermore, treating streptozotocin-induced diabetic rats with insulin restored SREBP-1 binding. In addition, we have identified an Sp1 binding site adjacent to the functional sterol-response element in the GLUT4 promoter. The Sp1 site appears to play an additive role in SREBP-1c mediated GLUT4 gene upregulation. These results suggest that upregulation of GLUT4 gene transcription might be directly mediated by SREBP-1c in adipose tissue. | - |
dc.language | English | - |
dc.publisher | PORTLAND PRESS LTD | - |
dc.subject | ELEMENT-BINDING PROTEIN-1C | - |
dc.subject | RESPONSIVE GLUCOSE-TRANSPORTER | - |
dc.subject | FATTY-ACID SYNTHESIS | - |
dc.subject | LIVER-X-RECEPTOR | - |
dc.subject | INSULIN ACTION | - |
dc.subject | DIABETES-MELLITUS | - |
dc.subject | SKELETAL-MUSCLE | - |
dc.subject | ADIPOSE-TISSUE | - |
dc.subject | MESSENGER-RNA | - |
dc.subject | PRETRANSLATIONAL SUPPRESSION | - |
dc.title | Regulation of GLUT4 gene expression by SREBP-1c in adipocytes | - |
dc.type | Article | - |
dc.identifier.wosid | 000240972900014 | - |
dc.identifier.scopusid | 2-s2.0-33749375790 | - |
dc.type.rims | ART | - |
dc.citation.volume | 399 | - |
dc.citation.beginningpage | 131 | - |
dc.citation.endingpage | 139 | - |
dc.citation.publicationname | BIOCHEMICAL JOURNAL | - |
dc.identifier.doi | 10.1042/BJ20060696 | - |
dc.contributor.localauthor | Kim, Hail | - |
dc.contributor.nonIdAuthor | IM, Seung-Soon | - |
dc.contributor.nonIdAuthor | Kwon, Sool-Ki | - |
dc.contributor.nonIdAuthor | Kang, Seung-Youn | - |
dc.contributor.nonIdAuthor | Kim, Tae-Hyun | - |
dc.contributor.nonIdAuthor | Hur, Man-Wook | - |
dc.contributor.nonIdAuthor | Kim, Kyung-Sup | - |
dc.contributor.nonIdAuthor | Ahn, Yong-Ho | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | adipocytes | - |
dc.subject.keywordAuthor | insulin | - |
dc.subject.keywordAuthor | sterol-response element | - |
dc.subject.keywordAuthor | sterol-regulatory-element-binding protein-1c (SREBP-1c) | - |
dc.subject.keywordAuthor | type 4 glucose transporter isoform (GLUT4) | - |
dc.subject.keywordPlus | ELEMENT-BINDING PROTEIN-1C | - |
dc.subject.keywordPlus | RESPONSIVE GLUCOSE-TRANSPORTER | - |
dc.subject.keywordPlus | FATTY-ACID SYNTHESIS | - |
dc.subject.keywordPlus | LIVER-X-RECEPTOR | - |
dc.subject.keywordPlus | INSULIN ACTION | - |
dc.subject.keywordPlus | DIABETES-MELLITUS | - |
dc.subject.keywordPlus | SKELETAL-MUSCLE | - |
dc.subject.keywordPlus | ADIPOSE-TISSUE | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | PRETRANSLATIONAL SUPPRESSION | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.