DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Yeon-Gil | ko |
dc.contributor.author | Raunser, Stefan | ko |
dc.contributor.author | Munger, Christine | ko |
dc.contributor.author | Wagner, John | ko |
dc.contributor.author | Song, Young-Lan | ko |
dc.contributor.author | Cygler, Miroslaw | ko |
dc.contributor.author | Walz, Thomas | ko |
dc.contributor.author | Oh, Byung-Ha | ko |
dc.contributor.author | Sacher, Michael | ko |
dc.date.accessioned | 2013-03-08T11:02:01Z | - |
dc.date.available | 2013-03-08T11:02:01Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2006-11 | - |
dc.identifier.citation | CELL, v.127, no.4, pp.817 - 830 | - |
dc.identifier.issn | 0092-8674 | - |
dc.identifier.uri | http://hdl.handle.net/10203/92876 | - |
dc.description.abstract | Transport protein particle (TRAPP) 1 is a multi-subunit vesicle tethering factor composed of seven subunits involved in ER-to-Golgi trafficking. The functional mechanism of the complex and how the subunits interact to form a functional unit are unknown. Here, we have used a multidisciplinary approach that includes X-ray crystallography, electron microscopy, biochemistry, and yeast genetics to elucidate the architecture of TRAPP 1. The complex is organized through lateral juxtaposition of the subunits into a flat and elongated particle. We have also localized the site of guanine nucleotide exchange activity to a highly conserved surface encompassing several subunits. We propose that TRAPP 1 attaches to Golgi membranes with its large flat surface containing many highly conserved residues and forms a platform for protein-protein interactions. This study provides the most comprehensive view of a multisubunit vesicle tethering complex to date, based on which a model for the function of this complex, involving Rab1-GTP and long, coiled-coil tethers, is presented. | - |
dc.language | English | - |
dc.publisher | CELL PRESS | - |
dc.subject | SPONDYLOEPIPHYSEAL DYSPLASIA-TARDA | - |
dc.subject | TO-GOLGI TRANSPORT | - |
dc.subject | CRYSTAL-STRUCTURE | - |
dc.subject | MEMBRANE-FUSION | - |
dc.subject | CIS-GOLGI | - |
dc.subject | IDENTIFICATION | - |
dc.subject | RECEPTOR | - |
dc.subject | DOCKING | - |
dc.subject | PROTEIN | - |
dc.subject | BET3 | - |
dc.title | The architecture of the multisubunit TRAPP I complex suggests a model for vesicle tethering | - |
dc.type | Article | - |
dc.identifier.wosid | 000242330600026 | - |
dc.identifier.scopusid | 2-s2.0-33750799403 | - |
dc.type.rims | ART | - |
dc.citation.volume | 127 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 817 | - |
dc.citation.endingpage | 830 | - |
dc.citation.publicationname | CELL | - |
dc.identifier.doi | 10.1016/j.cell.2006.09.029 | - |
dc.contributor.localauthor | Oh, Byung-Ha | - |
dc.contributor.nonIdAuthor | Kim, Yeon-Gil | - |
dc.contributor.nonIdAuthor | Raunser, Stefan | - |
dc.contributor.nonIdAuthor | Munger, Christine | - |
dc.contributor.nonIdAuthor | Wagner, John | - |
dc.contributor.nonIdAuthor | Song, Young-Lan | - |
dc.contributor.nonIdAuthor | Cygler, Miroslaw | - |
dc.contributor.nonIdAuthor | Walz, Thomas | - |
dc.contributor.nonIdAuthor | Sacher, Michael | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | SPONDYLOEPIPHYSEAL DYSPLASIA-TARDA | - |
dc.subject.keywordPlus | TO-GOLGI TRANSPORT | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | MEMBRANE-FUSION | - |
dc.subject.keywordPlus | CIS-GOLGI | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | DOCKING | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | BET3 | - |
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