Chelatococcus daeguensis sp nov., isolated from wastewater of a textile dye works, and emended description of the genus Chelatococcus

Abstract

A Gram-negative, non-spore-forming, rod-shaped bacterial strain, K106(T), was isolated from wastewater collected from a textile dye works in Korea. Strain K106(T) grew optimally at pH 7.0-7.5 and 30-37 degrees C in the presence of 0-1.0% (w/v) NaCl. A phylogenetic tree based on 16S rRNA gene sequences showed that strain K106(T) joined the type strain of Chelatococcus asaccharovorans with a bootstrap resampling value of 99.9%. The predominant ubiquinone of strain K106(T) was Q-10. The fatty acid profile of strain K106(T) was similar to that of C. asaccharovorans DSM 6462(T). Major polar lipids of strain K106(T) and C. asaccharovorans DSM 6462(T) were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, two aminolipids and two unidentified phospholipids. sym-Homospermidine, spermidine and putrescine were major polyamines. The DNA G + C content was 68.3 mol%. Strain K106(T) exhibited 16S rRNA gene sequence similarity of 96.6% to the type strain of C. asaccharovorans. DNA-DNA relatedness data and differential phenotypic properties, particularly differences in cell morphology and the ability to utilize nitrilotriacetate, demonstrated that strain K106(T) can be differentiated from C. asaccharovorans. On the basis of phenotypic, phylogenetic and genetic data, strain K106(T) represents a novel species of the genus Chelatococcus, for which the name Chelatococcus daeguensis sp. nov. is proposed. The type strain is K106(T) (=KCTC 12979(T) =CCUG 54519(T)).