Cellulomonas composti sp nov., a cellulolytic bacterium isolated from cattle farm compost

Abstract

A bacterial strain, TR706(T), which has cellulase and beta-glucosiclase activities, was isolated from compost at a cattle farm near Daejeon, Republic of Korea. It was a Gram-positive, aerobic or facultatively anaerobic, non-motile, rod-shaped bacterium. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belongs to the genus Cellulomonas, with highest sequence similarity to Gellulomonas uda DSM 20107(T) (98.5 %). Cell wall analysis revealed the presence of type A4 beta, L-Orn-D-Glu pepticloglycan. The cell-wall sugars detected were mannose and glucose. The predominant menaquinone was MK-9(H-4); MK-8(H-4) was detected in smaller quantities. The major fatty acids were anteiso-C-15:0, C-16:0, C-14:0 and C-18:0. The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The results of DNA-DNA hybridization and physiological and biochemical tests clearly demonstrated that TR7-06(T) represents a novel species. The combined genotypic and phenotypic data show that strain TR706(T) (= KCTC 19030(T) =NBRC 100758(T)) merits description as the type strain of a novel Cellulomonas species, Cellulomonas composti sp. nov.