Brevibacillus ginsengisoli sp nov., a denitrifying bacterium isolated from soil of a ginseng field

Abstract

A Gram-positive, rod-shaped, spore-forming bacterium, Gsoil 3088(T), was isolated from soil from a ginseng field in Pocheon Province in South Korea and characterized in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity, strain Gsoil 3088T was shown to belong to the family Paenibacillaceae, being related to Brevibacillus centrosporus (96.6 %), Brevibacillus borstelensis (96.3 %), Brevibacillus parabrevis (96.1 %), Brevibacillus formosus (96.1 %), Brevibacillus brevis (96.1 %) and Brevibacillus laterosporus (96.0%). The phylogenetic distances from other validly described species within the genus Brevibacillus were greater than 4.0 % (i.e. there was less than 96.0 % similarity). The G+C content of the genomic DNA was 52.1 mol%. Phenotypic and chemotaxonomic data (major menaquinone, MK-7; fatty acid profile, iSO-C-15:0, iso-C-14:0 and anteiso-C-15:0) supported the affiliation of strain Gsoil 3088(T) to the genus Brevibacillus. The results of physiological and biochemical tests allowed strain Gsoil 3088(T) to be distinguished genotypically and phenotypically from Brevibacillus species with validly published names. Strain Gsoil 3088(T), therefore, represents a novel species of the genus Brevibacillus, for which the name Brevibacillus ginsengisoli sp. nov. is proposed. The type strain is Gsoil 3088(T) (= KCTC 13938(T) =LMG 23403(T)).