DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sung, YH | ko |
dc.contributor.author | Lee, Gyun-Min | ko |
dc.date.accessioned | 2013-03-08T08:30:39Z | - |
dc.date.available | 2013-03-08T08:30:39Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2005-01 | - |
dc.identifier.citation | BIOTECHNOLOGY PROGRESS, v.21, pp.50 - 57 | - |
dc.identifier.issn | 8756-7938 | - |
dc.identifier.uri | http://hdl.handle.net/10203/92609 | - |
dc.description.abstract | When sodium butyrate (NaBu) was added to serum-free suspension culture of recombinant CHO (rCHO) cells for enhanced expression of human thrombopoietin (hTPO), apoptotic cell death of rCHO cells was induced in a dose-dependent manner and hTPO quality was deteriorated in regard to sialic acid and acidic isoform contents. To overcome these problems, we overexpressed Bcl-2 protein, an antiapoptotic protein, in rCHO cells producing hTPO. Compared to serum-free suspension culture of control cells without Bcl-2 overexpression (R-neo cells) and NaBu addition, a more than 10-fold increase in the maximum hTPO concentration was obtained in serum-free suspension culture of cells with Bcl-2 overexpression. (R-bcl2-14 cells) and 3 mM NaBu addition. Both the enhanced specific productivity endowed by NaBu and the extended culture longevity provided by the antiapoptotic effect of Bcl-2 overexpression contributed to the enhancement of maximum hTPO concentration. The problem of quality reduction of hTPO induced by NaBu was not solved by Bcl-2 overexpression, but it was not that significant. Compared to the culture in the absence of NaBu, the percentage of hTPO isoforms in pI 3-5 with high in vivo biological activity produced by R-bcl2-14 cells was decreased by approximately 18% in the presence of 3 mM. As a result, a more than 6-fold increase in the production of hTPO isoforms in pI 3-5 was achieved in R-bcl2-14 cell culture with 3 mM NaBu addition. Taken together, the data obtained suggest that Bcl-2 overexpression in rCHO cells and NaBu addition in serum-free suspension culture can be an effective means to enhance the production of highly glycosylated protein such as hTPO. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | HAMSTER OVARY CELLS | - |
dc.subject | BETA-GALACTOSIDE ALPHA-2,6-SIALYLTRANSFERASE | - |
dc.subject | MEGAKARYOCYTE GROWTH | - |
dc.subject | ANTIBODY-PRODUCTION | - |
dc.subject | BIOLOGICAL-ACTIVITY | - |
dc.subject | CHIMERIC ANTIBODY | - |
dc.subject | EXPRESSION | - |
dc.subject | APOPTOSIS | - |
dc.subject | GLYCOSYLATION | - |
dc.subject | LINE | - |
dc.title | Enhanced human thrombopoietin production by sodium butyrate addition to serum-free suspension culture of Bcl-2-overexpressing CHO cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000226934800008 | - |
dc.identifier.scopusid | 2-s2.0-13544271821 | - |
dc.type.rims | ART | - |
dc.citation.volume | 21 | - |
dc.citation.beginningpage | 50 | - |
dc.citation.endingpage | 57 | - |
dc.citation.publicationname | BIOTECHNOLOGY PROGRESS | - |
dc.contributor.localauthor | Lee, Gyun-Min | - |
dc.contributor.nonIdAuthor | Sung, YH | - |
dc.type.journalArticle | Article; Proceedings Paper | - |
dc.subject.keywordPlus | HAMSTER OVARY CELLS | - |
dc.subject.keywordPlus | BETA-GALACTOSIDE ALPHA-2,6-SIALYLTRANSFERASE | - |
dc.subject.keywordPlus | MEGAKARYOCYTE GROWTH | - |
dc.subject.keywordPlus | ANTIBODY-PRODUCTION | - |
dc.subject.keywordPlus | BIOLOGICAL-ACTIVITY | - |
dc.subject.keywordPlus | CHIMERIC ANTIBODY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | GLYCOSYLATION | - |
dc.subject.keywordPlus | LINE | - |
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