DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hwang, Su-Jeong | ko |
dc.contributor.author | Choi, Han-Ho | ko |
dc.contributor.author | Kim, Kyung-Tae | ko |
dc.contributor.author | Hong, Hyo-Jeong | ko |
dc.contributor.author | Koh, Gou-Young | ko |
dc.contributor.author | Lee, Gyun-Min | ko |
dc.date.accessioned | 2013-03-08T08:29:01Z | - |
dc.date.available | 2013-03-08T08:29:01Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2005-02 | - |
dc.identifier.citation | PROTEIN EXPRESSION AND PURIFICATION, v.39, no.2, pp.175 - 183 | - |
dc.identifier.issn | 1046-5928 | - |
dc.identifier.uri | http://hdl.handle.net/10203/92606 | - |
dc.description.abstract | Angiopoietin-2 (Ang2) is a complex regulator of vascular remodeling that plays a role in both blood vessel sprouting and blood vessel regression through its receptor Tie2. Recombinant Chinese hamster ovary (rCHO) cell lines expressing a high level (20 mug/mL) of recombinant human Ang2 protein (rhAng2) with an amino-terminal FLAG-tag was constructed by transfecting the expression vectors into dihydrofolate reductase (dhft)-deficient CHO cells and the subsequent gene amplification in medium containing step-wise increments in methotrexate level such as 0.02, 0.08, and 0.32 muM. The rhAng2 secreted from rCHO cells was purified at a purification yield of 53.6% from the cultured medium using an anti-FLAG M2 agarose affinity gel. SDS-PAGE and Western blot analyses showed, that rCHO cells secret rhAng2 as a homodimeric glycoprotein form. Furthermore. rhAng2 binds to the Tie2 receptor and phosphorylates Tie2 in a concentration-dependent manner. Therefore. our rhAng2 could be useful for clarifying biological effect of exogenous Ang2 in the future. (C) 2004 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | SIGNAL-TRANSDUCTION PATHWAY | - |
dc.subject | MEDIATED GENE AMPLIFICATION | - |
dc.subject | RECEPTOR TYROSINE KINASES | - |
dc.subject | ENDOTHELIAL GROWTH-FACTOR | - |
dc.subject | CHO-CELLS | - |
dc.subject | MAMMALIAN-CELLS | - |
dc.subject | TIE2 RECEPTOR | - |
dc.subject | ANGIOGENESIS | - |
dc.subject | SURVIVAL | - |
dc.subject | DOMAINS | - |
dc.title | Expression and purification of recombinant human angiopoietin-2 produced in Chinese hamster ovary cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000226437000007 | - |
dc.identifier.scopusid | 2-s2.0-11844287623 | - |
dc.type.rims | ART | - |
dc.citation.volume | 39 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 175 | - |
dc.citation.endingpage | 183 | - |
dc.citation.publicationname | PROTEIN EXPRESSION AND PURIFICATION | - |
dc.contributor.localauthor | Koh, Gou-Young | - |
dc.contributor.localauthor | Lee, Gyun-Min | - |
dc.contributor.nonIdAuthor | Choi, Han-Ho | - |
dc.contributor.nonIdAuthor | Kim, Kyung-Tae | - |
dc.contributor.nonIdAuthor | Hong, Hyo-Jeong | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | angiopoietin | - |
dc.subject.keywordAuthor | FLAG-tag | - |
dc.subject.keywordAuthor | Tie2 receptor | - |
dc.subject.keywordAuthor | Chinese hamster ovary cells | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION PATHWAY | - |
dc.subject.keywordPlus | MEDIATED GENE AMPLIFICATION | - |
dc.subject.keywordPlus | RECEPTOR TYROSINE KINASES | - |
dc.subject.keywordPlus | ENDOTHELIAL GROWTH-FACTOR | - |
dc.subject.keywordPlus | CHO-CELLS | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | TIE2 RECEPTOR | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | SURVIVAL | - |
dc.subject.keywordPlus | DOMAINS | - |
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