DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이정아 | ko |
dc.contributor.author | 박세일 | ko |
dc.contributor.author | 이승섭 | ko |
dc.contributor.author | 곽주현 | ko |
dc.contributor.author | 이광철 | ko |
dc.contributor.author | 황성필 | ko |
dc.date.accessioned | 2013-03-08T05:58:49Z | - |
dc.date.available | 2013-03-08T05:58:49Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2007-10 | - |
dc.identifier.citation | 대한기계학회논문집 A, v.31, no.10, pp.986 - 992 | - |
dc.identifier.issn | 1226-4873 | - |
dc.identifier.uri | http://hdl.handle.net/10203/92286 | - |
dc.description.abstract | This paper presents the fabrication and characterization of carbon films pyrolyzed with various photoresists for bioMEMS applications. To verify the usefulness of pyrolyzed carbon films as an electrode material in an electrochemical biosensor developed by the authors, interactions between avidin and biotin on the pyrolyzed carbon film were studied via electrochemical impedance spectroscopy based on electrostatic interactions between avidin and negatively-charged ferricyanide. The pyrolyzed carbon films were characterized using a surface profiler, a precision semiconductor parameter analyzer, a nanoindentor, scanning electron microscopy, and atomic force microscopy. Amine conjugated biotin was immobilized on the electrode using EDC/NHS as crosslinkers after O2 plasma treatment to enhance functional groups on the carbon electrode pyrolyzed at $1000^{\circ}C$ with AZ9260. The detection of avidin binding with different concentrations in a range of 0.75 nM to $7.5\;{\mu}M$ to the pyrolyzed carbon electrode modified with biotin was carried out by measuring the electrochemical impedance change. The results show that avidin binds to the biotin on the electrode not by non-specific interaction but by specific interaction, and that EIS successfully detects this binding event. Pyrolyzed carbon films are a promising material for miniaturization, integration, and low-cost fabrication in electrochemical biosensors. | - |
dc.language | Korean | - |
dc.publisher | 대한기계학회 | - |
dc.title | 전기화학 바이오센서의 전극물질로 응용을 위한 열분해 탄소의 제작 및 특성연구 | - |
dc.title.alternative | Fabrication and Characterization of Pyrolyzed Carbon for Use as an Electrode Material in Electrochemical Biosensor | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.citation.volume | 31 | - |
dc.citation.issue | 10 | - |
dc.citation.beginningpage | 986 | - |
dc.citation.endingpage | 992 | - |
dc.citation.publicationname | 대한기계학회논문집 A | - |
dc.identifier.kciid | ART001081826 | - |
dc.contributor.localauthor | 이승섭 | - |
dc.contributor.nonIdAuthor | 이정아 | - |
dc.contributor.nonIdAuthor | 박세일 | - |
dc.contributor.nonIdAuthor | 곽주현 | - |
dc.contributor.nonIdAuthor | 이광철 | - |
dc.contributor.nonIdAuthor | 황성필 | - |
dc.subject.keywordAuthor | Biosensor(바이오센서) | - |
dc.subject.keywordAuthor | Electrochemical Impedance Spectroscopy(전기화학 임피던스 분광학) | - |
dc.subject.keywordAuthor | MEMS(멤스) | - |
dc.subject.keywordAuthor | Pyrolyzed Carbon Electrode(열분해 탄소전극) | - |
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