DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, DW | ko |
dc.contributor.author | Cho, Young-Ho | ko |
dc.date.accessioned | 2013-03-08T03:03:47Z | - |
dc.date.available | 2013-03-08T03:03:47Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2007-06 | - |
dc.identifier.citation | SENSORS AND ACTUATORS B-CHEMICAL, v.124, pp.84 - 89 | - |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.uri | http://hdl.handle.net/10203/91932 | - |
dc.description.abstract | We present a continuous and low voltage cell lysis device in which a width and length of a channel change to generate focused the high electric field strength for cell lysis and the low electric field strength for a transport of samples. The previous cell lysis devices acquire the high electric field strength for a cell lysis by applying an ac voltage to a micro-gap between electrodes and require additional pumps or valves for a sample transport. However, when we change the width and length of the channel between a pair of external electrodes attached to a dc voltage, we generate both the high electric field strength for a cell lysis and the low electric field strength for an electroosmotic flow. The present device therefore performs continuous cell lysis and a sample transport without needing either an additional flow source or an additional process fabricating the electrodes for the micro-gap. The experimental study features an orifice whose width and length is 20 times narrower and 175 times shorter than the width and length of a microchannel. With an operational voltage of 50 V, the present device generates high electric field strength of 1.2 kV/cm at the orifice to disrupt cells with 100% lysis rate of red blood cells and low electric field strength of 60 V/cm at the microchannel to generate an electroosmotic flow of 30 +/- 9 mu m/s. In conclusion, the present device is capable of continuous self-pumping cell lysis at a low voltage; thus, it is suitable for a sample pretreatment component of a micro total analysis system or lab-on-a-chip. (c) 2006 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE SA | - |
dc.subject | CHIP | - |
dc.subject | MANIPULATION | - |
dc.title | A continuous electrical cell lysis device using a low dc voltage for a cell transport and rupture | - |
dc.type | Article | - |
dc.identifier.wosid | 000247261900013 | - |
dc.identifier.scopusid | 2-s2.0-34248579697 | - |
dc.type.rims | ART | - |
dc.citation.volume | 124 | - |
dc.citation.beginningpage | 84 | - |
dc.citation.endingpage | 89 | - |
dc.citation.publicationname | SENSORS AND ACTUATORS B-CHEMICAL | - |
dc.identifier.doi | 10.1016/j.snb.2006.11.054 | - |
dc.contributor.localauthor | Cho, Young-Ho | - |
dc.contributor.nonIdAuthor | Lee, DW | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | cell lysis | - |
dc.subject.keywordAuthor | electroosmosis | - |
dc.subject.keywordAuthor | electroosmotic pump | - |
dc.subject.keywordAuthor | electric field modulation | - |
dc.subject.keywordPlus | CHIP | - |
dc.subject.keywordPlus | MANIPULATION | - |
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