DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, BR | ko |
dc.contributor.author | Lee, Younghoon | ko |
dc.date.accessioned | 2013-03-07T19:47:30Z | - |
dc.date.available | 2013-03-07T19:47:30Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2005-12 | - |
dc.identifier.citation | BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.26, no.12, pp.2033 - 2037 | - |
dc.identifier.issn | 0253-2964 | - |
dc.identifier.uri | http://hdl.handle.net/10203/91129 | - |
dc.description.abstract | In vitro selection was used to isolate Mg2+-dependent self-cleaving ribozymes with cis-cleavage activity from a pre-tRNA library having 40-mer random sequences attached to 5'-end of E coli tRNA(Phe). After 8 rounds of SELEX (Systematic Evolution of Ligands by Exponential Enrichment), RNA molecules which can self-cleave at the high concentration of Mg2+ were isolated. The selected ribozymes can carry out the self-cleavage reaction in the presence of 100 mM Mg2+ but not in 10 MM Mg2+. The cleavage sites of the ribozymes are located at +3 and +4 of tRNA(Phe), compared with +1 position of 5'-end cleavage site of pre-tRNA by RNase P. New RNA constructs deprived of its D stem-loop, anticodon stem-loop, variable loop and T stern-loop, respectively showed the cleavage specificity identical to a ribozyme having the intact tRNA structure. Also, the new ribozyme fused with both a ribozyme and tRNA(Leu) showed the cleavage activities at the various sites within its sequences, different from two sites of position +3 and +4 observed in the ribozyme with tRNA(Phe). Our results suggest that the selected ribozyme is not structural-specific for tRNA. | - |
dc.language | English | - |
dc.publisher | KOREAN CHEMICAL SOC | - |
dc.subject | RIBONUCLEASE-P | - |
dc.subject | TRANSFER-RNA | - |
dc.subject | DIVERSITY | - |
dc.subject | BINDING | - |
dc.subject | PROTEIN | - |
dc.title | Isolation of new self-cleaving ribozymes with in vitro selection | - |
dc.type | Article | - |
dc.identifier.wosid | 000234694600026 | - |
dc.identifier.scopusid | 2-s2.0-31744439492 | - |
dc.type.rims | ART | - |
dc.citation.volume | 26 | - |
dc.citation.issue | 12 | - |
dc.citation.beginningpage | 2033 | - |
dc.citation.endingpage | 2037 | - |
dc.citation.publicationname | BULLETIN OF THE KOREAN CHEMICAL SOCIETY | - |
dc.contributor.localauthor | Lee, Younghoon | - |
dc.contributor.nonIdAuthor | Cho, BR | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | SELEX | - |
dc.subject.keywordAuthor | self-cleavage ribozyme | - |
dc.subject.keywordAuthor | tRNA | - |
dc.subject.keywordAuthor | H1 RNA | - |
dc.subject.keywordPlus | RIBONUCLEASE-P | - |
dc.subject.keywordPlus | TRANSFER-RNA | - |
dc.subject.keywordPlus | DIVERSITY | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | PROTEIN | - |
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