Direct force measurements of biomolecular interactions by nanomechanical force gauge

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Without using the laser and optical detection method of an atomic force microscope (AFM), direct force measurements of biomolecular interactions in biological solution are accomplished by a nanomechanical force gauge. The device consists of integrated nanoscale single-crystal Si cantilever and reading scales, which allow the direct measurements of biomolecular interactions by reading the deflection of the cantilever with an optical microscope. The spring constant of the cantilevers was calibrated by measuring the resonant frequency under electrostatic force excitation, and the minimum value among the designed devices shows 80 pN/mu m. The piconewton regime dissociation force between a biotinylated bead and streptavidins in an aqueous solution was directly measured with this device. (c) 2005 American Institute of Physics.
Publisher
AMER INST PHYSICS
Issue Date
2005-05
Language
English
Article Type
Article
Citation

APPLIED PHYSICS LETTERS, v.86, no.19

ISSN
0003-6951
DOI
10.1063/1.1906332
URI
http://hdl.handle.net/10203/91113
Appears in Collection
BiS-Journal Papers(저널논문)
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