DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jeong-Gun | ko |
dc.contributor.author | Yun, Kyusik | ko |
dc.contributor.author | Lim, GS | ko |
dc.contributor.author | Lee, SE | ko |
dc.contributor.author | Kim, S | ko |
dc.contributor.author | Park, Je-Kyun | ko |
dc.date.accessioned | 2013-03-07T18:04:26Z | - |
dc.date.available | 2013-03-07T18:04:26Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2007-05 | - |
dc.identifier.citation | BIOELECTROCHEMISTRY, v.70, pp.228 - 234 | - |
dc.identifier.issn | 1567-5394 | - |
dc.identifier.uri | http://hdl.handle.net/10203/90861 | - |
dc.description.abstract | This paper reports a novel detection method for DNA hybridization based on the electrochemiluminescence (ECL) of Ru(bpy)(3)(2+) with a DNA-binding intercalator as a reductant of Ru(bpy)(3)(3+). Some ECL-inducible intercalators; have been screened in this study using electrochemical methods combined with a chemiluminescent technique. The double-stranded DNA intercalated by doxorubicin, daunorubicin, or 4 ',6-diamidino-2-phenylindole (DAPI) shows a good ECL with Ru(bpy)(3)(2+) at + 1.19 V (versus Ag/AgCl), while the non-intercalated single-stranded DNA does not. In order to stabilize the self-assembled DNA molecules during ECL reaction, we constructed the ECL DNA biosensor separating the ECL working electrode with an immobilized DNA probe. A gold electrode array on a plastic plate was assembled with a thru-hole array where oligonucleotide probes were immobilized in the side wall of thru-hole array. The fabricated ECL DNA biosensor was used to detect several pathogens using ECL technique. A good specificity of single point mutations for hepatitis disease was obtained by using the DAPI-intercalated Ru (bpy)(3)(2+) ECL. (C) 2006 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE SA | - |
dc.subject | ELECTROGENERATED CHEMILUMINESCENCE | - |
dc.subject | HYBRIDIZATION | - |
dc.subject | SYSTEM | - |
dc.subject | ABEI | - |
dc.subject | ECL | - |
dc.title | DNA biosensor based on the electrochemiluminescence of Ru(bpy)(3)(2+) with DNA-binding intercalators | - |
dc.type | Article | - |
dc.identifier.wosid | 000246254500005 | - |
dc.identifier.scopusid | 2-s2.0-33947721147 | - |
dc.type.rims | ART | - |
dc.citation.volume | 70 | - |
dc.citation.beginningpage | 228 | - |
dc.citation.endingpage | 234 | - |
dc.citation.publicationname | BIOELECTROCHEMISTRY | - |
dc.identifier.doi | 10.1016/j.bioelechem.2006.09.003 | - |
dc.contributor.localauthor | Park, Je-Kyun | - |
dc.contributor.nonIdAuthor | Lee, Jeong-Gun | - |
dc.contributor.nonIdAuthor | Yun, Kyusik | - |
dc.contributor.nonIdAuthor | Lim, GS | - |
dc.contributor.nonIdAuthor | Lee, SE | - |
dc.contributor.nonIdAuthor | Kim, S | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | electrochemiluminescence (ECL) | - |
dc.subject.keywordAuthor | tris-(2,2 &apos | - |
dc.subject.keywordAuthor | -bipyridyl)ruthenium(II) | - |
dc.subject.keywordAuthor | DNA-binding intercalator | - |
dc.subject.keywordAuthor | 4 &apos | - |
dc.subject.keywordAuthor | ,6-diamidino-2-phenylindole (DAPI) | - |
dc.subject.keywordAuthor | DNA biosensor | - |
dc.subject.keywordAuthor | DNA hybridization detection | - |
dc.subject.keywordPlus | ELECTROGENERATED CHEMILUMINESCENCE | - |
dc.subject.keywordPlus | HYBRIDIZATION | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | ABEI | - |
dc.subject.keywordPlus | ECL | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.