DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jang, Moon-Sun | ko |
dc.contributor.author | Sul, Jee-Won | ko |
dc.contributor.author | Choi, Byung-Jung | ko |
dc.contributor.author | Lee, Su-Jin | ko |
dc.contributor.author | Suh, Jee-Hee | ko |
dc.contributor.author | Kim, Nam-Soon | ko |
dc.contributor.author | Kim, Woo Ho | ko |
dc.contributor.author | Lim, Dae-Sik | ko |
dc.contributor.author | Lee, Chang-Woo | ko |
dc.contributor.author | Kim, Eunhee | ko |
dc.date.accessioned | 2013-03-07T14:44:42Z | - |
dc.date.available | 2013-03-07T14:44:42Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2008-11 | - |
dc.identifier.citation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.283, no.47, pp.32344 - 32351 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/10203/90423 | - |
dc.description.abstract | This study reports that Aurora-A (Aur-A) phosphorylates Fas-associated factor-1 (FAF1) at Ser-289 and Ser-291. Forced expression of a FAF1 mutant mimicking phosphorylation at Ser-289 and Ser-291 (FAF1 DD), but not phosphorylation-deficient FAF1 (FAF1 AA), reduced Aur-A expression. However, transfection of FAF1 DD failed to reduce Aur-A expression in the presence of MG132 and MG115, indicating that this decrease is proteasome-mediated. Additionally, transfection of FAF1 DD suppressed the expression of Aur-A in ts20-BALB cells lacking E1 ubiquitin (Ub) activating enzyme activity at restrictive temperatures and also reduced the expression of Aur-A S51D, a mutant resistant to Ub-dependent degradation. Our data indicate that phosphorylated FAF1 mediates the ubiquitin-independent, proteasome-dependent degradation of Aur-A. Overexpression of FAF1 DD blocked Aur-A-induced centrosome amplification and accumulated cells in G(2)/M phase, representing cellular phenotypes consistent with the anticipated loss of Aur-A. Collectively, our findings support the negative feedback regulation of Aur-A via phosphorylation of the death-promoting protein, FAF1, and disclose the presence of molecular cross-talk between constituents of the cell cycle and cell death machinery. | - |
dc.language | English | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.subject | PROTEIN-KINASE CK2 | - |
dc.subject | CENTROSOME AMPLIFICATION | - |
dc.subject | INDUCED APOPTOSIS | - |
dc.subject | FACTOR FAF1 | - |
dc.subject | KAPPA-B | - |
dc.subject | OVEREXPRESSION | - |
dc.subject | DEGRADATION | - |
dc.subject | INTERACTS | - |
dc.subject | PATHWAY | - |
dc.subject | COMPLEX | - |
dc.title | Negative Feedback Regulation of Aurora-A via Phosphorylation of Fas-associated Factor-1 | - |
dc.type | Article | - |
dc.identifier.wosid | 000260893700021 | - |
dc.identifier.scopusid | 2-s2.0-57749092974 | - |
dc.type.rims | ART | - |
dc.citation.volume | 283 | - |
dc.citation.issue | 47 | - |
dc.citation.beginningpage | 32344 | - |
dc.citation.endingpage | 32351 | - |
dc.citation.publicationname | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.identifier.doi | 10.1074/jbc.M804199200 | - |
dc.contributor.localauthor | Lim, Dae-Sik | - |
dc.contributor.nonIdAuthor | Jang, Moon-Sun | - |
dc.contributor.nonIdAuthor | Sul, Jee-Won | - |
dc.contributor.nonIdAuthor | Choi, Byung-Jung | - |
dc.contributor.nonIdAuthor | Lee, Su-Jin | - |
dc.contributor.nonIdAuthor | Suh, Jee-Hee | - |
dc.contributor.nonIdAuthor | Kim, Nam-Soon | - |
dc.contributor.nonIdAuthor | Kim, Woo Ho | - |
dc.contributor.nonIdAuthor | Lee, Chang-Woo | - |
dc.contributor.nonIdAuthor | Kim, Eunhee | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | PROTEIN-KINASE CK2 | - |
dc.subject.keywordPlus | CENTROSOME AMPLIFICATION | - |
dc.subject.keywordPlus | INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | FACTOR FAF1 | - |
dc.subject.keywordPlus | KAPPA-B | - |
dc.subject.keywordPlus | OVEREXPRESSION | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | INTERACTS | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | COMPLEX | - |
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