DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yim, Se-Jeong | ko |
dc.contributor.author | Lee, Yong-Seok | ko |
dc.contributor.author | Lee, Jin-A | ko |
dc.contributor.author | Chang, Deok-Jin | ko |
dc.contributor.author | Han, Jin-Hee | ko |
dc.contributor.author | Kim, Hyoung | ko |
dc.contributor.author | Park, Hyungju | ko |
dc.contributor.author | Jun, Heejung | ko |
dc.contributor.author | Kim, V. Narry | ko |
dc.contributor.author | Kaang, Bong-Kiun | ko |
dc.date.accessioned | 2013-03-07T13:59:07Z | - |
dc.date.available | 2013-03-07T13:59:07Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2006-07 | - |
dc.identifier.citation | JOURNAL OF NEUROCHEMISTRY, v.98, no.2, pp.420 - 429 | - |
dc.identifier.issn | 0022-3042 | - |
dc.identifier.uri | http://hdl.handle.net/10203/90348 | - |
dc.description.abstract | Aplysia CCAAT enhancer-binding protein (ApC/EBP), a key molecular switch in 5-hydroxytryptamine (5-HT)-induced long-term facilitation of Aplysia, is quickly and transiently expressed in response to a 5-HT stimulus, but the mechanism underlying this dynamic expression profile remains obscure. Here, we report that the dynamic expression of ApC/EBP during long-term facilitation is regulated at the post-transcriptional level by AU-rich element (ARE)-binding proteins. We found that the 3'UTR of ApC/EBP mRNA contains putative sequences for ARE, which is a representative post-transcriptional cis-acting regulatory element that modulates the stability and/or the translatability of a distinct subset of labile mRNAs. We cloned the Aplysia homologue of embryonic lethal abnormal visual system homologue (ELAV/Hu) protein, one of the best-studied RNA-binding proteins that associate with ARE, and elucidated the involvement of Aplysia ELAV/Hu protein in ApC/EBP gene expressional regulation. Cloned Aplysia ELAV/Hu protein, Aplysia embryonic lethal abnormal visual system (ApELAV), bound to an AU-rich region within the 3'UTR of ApC/EBP mRNA. Additionally, ApELAV controlled the expression of ApC/EBP 3'UTR-containing reporter gene by functioning as a stability-enhancing factor. In particular, 5-HT-induced long-term facilitation was impaired when the AU-rich region within the 3'UTR of ApC/EBP was over-expressed, which suggests the significance of this region in 5-HT-induced ApC/EBP expression, and in the resultant formation of long-term facilitation. Our results imply that the Aplysia ARE-binding protein, ApELAV, can regulate ApC/EBP gene expression at the mRNA level, and accordingly, ARE-mediated post-transcriptional mechanism may serve a crucial function in regulating the expression of ApC/EBP in response to a 5-HT stimulus. | - |
dc.language | English | - |
dc.publisher | Wiley-Blackwell | - |
dc.subject | ELAV-LIKE PROTEIN | - |
dc.subject | POSTTRANSCRIPTIONAL REGULATION | - |
dc.subject | NEURONAL DIFFERENTIATION | - |
dc.subject | SELECTIVE DEGRADATION | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | NERVOUS-SYSTEM | - |
dc.subject | UP-REGULATION | - |
dc.subject | HUR | - |
dc.subject | STABILITY | - |
dc.subject | LOCALIZATION | - |
dc.title | Regulation of ApC/EBP mRNA by the Aplysia AU-rich element-binding protein, ApELAV, and its effects on 5-hydroxytryptamine-induced long-term facilitation | - |
dc.type | Article | - |
dc.identifier.wosid | 000238487000011 | - |
dc.identifier.scopusid | 2-s2.0-33746515063 | - |
dc.type.rims | ART | - |
dc.citation.volume | 98 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 420 | - |
dc.citation.endingpage | 429 | - |
dc.citation.publicationname | JOURNAL OF NEUROCHEMISTRY | - |
dc.identifier.doi | 10.1111/j.1471-4159.2006.03887.x | - |
dc.contributor.localauthor | Han, Jin-Hee | - |
dc.contributor.nonIdAuthor | Yim, Se-Jeong | - |
dc.contributor.nonIdAuthor | Lee, Yong-Seok | - |
dc.contributor.nonIdAuthor | Lee, Jin-A | - |
dc.contributor.nonIdAuthor | Chang, Deok-Jin | - |
dc.contributor.nonIdAuthor | Kim, Hyoung | - |
dc.contributor.nonIdAuthor | Park, Hyungju | - |
dc.contributor.nonIdAuthor | Jun, Heejung | - |
dc.contributor.nonIdAuthor | Kim, V. Narry | - |
dc.contributor.nonIdAuthor | Kaang, Bong-Kiun | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | CCAAT enhancer-binding protein | - |
dc.subject.keywordAuthor | Aplysia | - |
dc.subject.keywordAuthor | AU-rich element (ARE)-binding protein | - |
dc.subject.keywordAuthor | long-term facilitation | - |
dc.subject.keywordAuthor | memory | - |
dc.subject.keywordAuthor | synaptic plasticity | - |
dc.subject.keywordPlus | ELAV-LIKE PROTEIN | - |
dc.subject.keywordPlus | POSTTRANSCRIPTIONAL REGULATION | - |
dc.subject.keywordPlus | NEURONAL DIFFERENTIATION | - |
dc.subject.keywordPlus | SELECTIVE DEGRADATION | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | NERVOUS-SYSTEM | - |
dc.subject.keywordPlus | UP-REGULATION | - |
dc.subject.keywordPlus | HUR | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | LOCALIZATION | - |
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