DC Field | Value | Language |
---|---|---|
dc.contributor.author | 정유연 | ko |
dc.contributor.author | 최철희 | ko |
dc.date.accessioned | 2013-03-07T13:05:32Z | - |
dc.date.available | 2013-03-07T13:05:32Z | - |
dc.date.created | 2012-04-04 | - |
dc.date.created | 2012-04-04 | - |
dc.date.issued | 2005-02 | - |
dc.identifier.citation | ANNUAL BULLETIN OF THE BUM-SUK ACADEMIC SCHOLARSHIP FOUNDATION, v.9, pp.43 - 54 | - |
dc.identifier.issn | 1229-2591 | - |
dc.identifier.uri | http://hdl.handle.net/10203/90239 | - |
dc.description.abstract | Purpose: The purpose of this study is to determine the molecular mechanism responsible for the anti-tumor effect of PPARg ligands. Materials and Methods: Apoptosis was measured by flow cytometric analysis and mitochondrial transmembrane potential was determined using an inverted epifluorescence microscope and FACS analysis after staining with JC-1 and TMRE. Intracellular levels of reactive oxygen species (ROS) were determined after staining with DCF-DA. Results: PPARg ligands (troglitazone, ciglitazone and 15d-PGJ2) induced apoptotic cell death of astrocytoma cells but not human primary cultured fetal astrocyte. PPARg ligands generated intracellular ROS within 1 h and subsequentlt induced depolarization of mitochondrial transmembrane and apoptosis. PPARg ligands-induced mitochondrial depolarization and apoptosis were suppressed by pretreatment with ROS scavengers, NAC and EGCG. These results collectively indicate that a PPARg ligands-induced apoptosis is mediated by generation of ROS and subsequent depolarization of mitochondrial transmembrane potential and caspase 3 activation. Conclusion: Our results suggest that PPARg ligands, antidiabetic drugs, might be utilized as an effective anti-cancer therapy for treatment of highly malignant astrocytoma. | - |
dc.language | Korean | - |
dc.publisher | 범석학술재단 | - |
dc.title | Effect of PPAR gamma ligands on cell death of astrocytoma cells | - |
dc.title.alternative | 범석학술재단 | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.citation.volume | 9 | - |
dc.citation.beginningpage | 43 | - |
dc.citation.endingpage | 54 | - |
dc.citation.publicationname | ANNUAL BULLETIN OF THE BUM-SUK ACADEMIC SCHOLARSHIP FOUNDATION | - |
dc.contributor.localauthor | 최철희 | - |
dc.contributor.nonIdAuthor | 정유연 | - |
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